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Rat IgG2a Isotype Control Isotype Control – Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS679088

MBS679088 is an isotype control targeting Rat IgG2a Isotype Control. Also known as Rat IgG2a-UNLB. Host: Rat. More details

$255 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rat IgG2a Isotype Control
Full Product Name
Rat IgG2a-UNLB
Product Synonym Names
Rat IgG2a - Purified, Unlabeled Antibody; Rat IgG2a Isotype Control
Isotype
Rat IgG2a kappa
Clone Number
KLH/G2a-1-1
Host
Rat
Form/Format
Purified (UNLB) Antibody
Specificity
Keyhole limpet hemocyanin (KLH)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium Azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Characterization
To insure lot-to-lot consistency, each batch of product is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
Applications Tested/Suitable for Rat IgG2a Isotype Control isotype control
ELISA; Flow Cytometry
WORKING DILUTIONS

Flow Cytometry
Flourescein, AF488, and PacBlu conjugates: < or = ug/10^6 cells
Biotin conjugate: < or = ug/10^6 cells
R-phycoerythrin conjugate: < or = 0.2 ug/10^6 cells
R-phycoerythrin conjugate-Texas Red^(R): < or = 0.2 ug/10^6 cells
Allophycocyanin conjugate: < or = 0.2 ug/10^6 cells
Spectral Red^TM conjugate: < or = 0.2 ug/10^6 cells
Cyanine 5 conjugate: < or = 0.2 ug/10^6 cells
PE/CY5.5 and PE/CY7 conjugates: < or = 0.2 ug/10^6 cells
APC/CY5.5 and APC/CY7 conjugates: < or = 0.2 ug/10^6 cells
AF647 conjugate: < or = 0.2 ug/10^6 cells

Other Notes

Small volumes of Rat IgG2a Isotype Control isotype control vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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