MyBioSource Life Science Resources
ELISA Testing
ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.
Western Blotting
Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes.
Southern Blotting
The first of these techniques developed was the Southern blot, named after Dr. Edwin Southern who developed it to identify specific DNA sequences. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. The process starts from electrophoresis of DNA molecules which are hybridized in a blotting membrane followed by a transfer step where DNA from gel is transferred onto the blotting membrane.
Gene Knockout
When a mutation inactivates a gene function it is called as gene knockout. Gene knockout has its application both in classical genetics and modern techniques such as functional genomics. During the initial timescale, gene knockout was carried n transposon mutagenesis. The major disadvantage of that method is the laborious screening to find the knocked out gene.
Branched DNA Assay
The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by boosting the reporter signal, rather than replicating target sequences as the means of detection, and hence avoids the errors inherent in the extraction and amplification of target sequences.
Polymerase Chain Reaction
PCR is a technology which has its application in all molecular biology applications. The advent of PCR meant that insufficiencies in the quantity of DNA were no longer a limitation in molecular biology research or diagnostic procedures. The chemistry involved in PCR depends on the complementarity (matching) of the nucleotide bases in the double-stranded DNA helix. When a molecule of DNA is sufficiently heated, the hydrogen bonds holding together the double helix are disrupted and the molecule separates or denatures into single strands. If the DNA solution is allowed to cool, the complementary base pairs can reform to restore the original double helix.
Gene Transfer Technique
Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale experiment. However, the techniques of gene transfer in bacteria like Escherichia coli (E. coli) are simple and appropriate for the teaching and learning laboratory. Plasmid-based genetic transformation is the most common used technology for genetic transfer.
Recent Publications
A Guide To The History Of Antibiotics
Table of Content 1. Introduction 2. What are antibiotics? A. How antibiotics work and their limitations B. When antibiotics are commonly used C. Limitations of antibiotics 3. History of antibiotics A. Ancient antibiotics B. Before modern antibiotics C. Before modern...
What Is DNA? Everything You Need To Know
Table of Contents 1. Introduction A. Historical contributions to science B. Modern advances in medicine C. Importance of GoFR D. Benefits and concerns of GoFR 2. What is Gain of Function research? 3. What is the purpose of Gain of Function research? A. How GoFR allows...
How Did Humans Evolve? A Complete Guide To Human Evolution
Table of Content 1. Introduction 2. What were early humans like? A. The emergence of Homo habilis B. The lifestyle of Homo Habilis C. The significance of Homo Habilis in Human evolution 3. How long have humans existed? A. The emergence of Homo sapiens B. Significance...
What Is A Gene? A Complete Guide To Genes
Table of Contents 1. Introduction to Genes and DNA 2. What is a Gene? A. The purpose and complexity of Genes B. Alleles and Phenotypes C. Gene Variants and Feature Expression A. Individuality and Family History 3. What is genetic mutation A. Causes of genetic...
A History Of Viruses And Bacteria (And The Worst Of Them)
Table of Contents What is Virus? Transmission of Viruses Contagious period after contracting a viral infection Can the Virus evolve? Types of Viruses Some of the common viral diseases What is Bacteria? Difference between a Virus and a Bacteria Reproduction of bacteria...
A Guide To Genetic Engineering Biotech And How It Works
Table of Contents Introduction What is genetic engineering The benefits of genetic engineering Concerns surrounding genetic engineering What is the genetic engineering used for Improved crop yield and quality Reduced use of pesticides and herbicides Producing...
The Future of Cloning: From Dolly The Sheep To Elizabeth Ann
Table of Contents 1. Introduction 2. Is Cloning possible? o Stem cell research and cloning o Cloning whole organisms 3. Do Clones ever occur naturally? o Asexual reproduction in single-celled organisms 4. The types of artificial cloning o Gene...
National Poison Prevention Week
Table of Contents I. Introduction • Accidental poisonings as a leading public health problem II. Factors Contributing to Pediatric Poisoning • Developmental milestones sequence • Child-associated, poison-associated, and environment-associated factors • Risk factors...
World TB Day
Table of Contents 1. Introduction • Background and history of tuberculosis (TB) • Global impact of TB • World TB Day 2. Pathogenesis of TB • How TB is acquired and its prevalence • Target host cell and immune response • Molecular structure of M. tuberculosis •...
HIV/AIDS Awareness Day
Table of Contents 1. Introduction • Brief history of HIV infection 2. Understanding HIV 3. Fighting HIV/AIDS • Importance of stopping HIV replication in T-cells • Current treatments for HIV/AIDS 4. Living with HIV/AIDS • Symptoms of HIV infection • Importance of...
American Diabetes Alert Day
Table of Contents Diabetes in the US American Diabetes Alert Day Understanding Diabetes Types of Diabetes Testing for Diabetes Complications of Diabetes Prevention and Management of Diabetes Diabetes affects about 9.4 percent of the U.S. population. A...
Epilepsy Awareness Day
Table of Contents Diabetes in the US American Diabetes Alert Day Understanding Diabetes Types of Diabetes Testing for Diabetes Complications of Diabetes Prevention and Management of Diabetes Epilepsy which is a neurological condition affecting the nervous system, also...
Brain Awareness Week
Table of Contents I. Overview of the complexity and functions of the brain II. Anatomy of the Brain A.Ventricles and support cells B. Divisions of the nervous system III. The Cerebrum A. Hemispheres and corpus callosum B. Lobes and associated functions IV. The...
National Sleep Awareness Week
Table of Contents I. Introduction • Importance of sleep for physical and mental health • National Sleep Awareness Week and its theme • Recommended hours of sleep per day II. Common Sleep Disorders III. Guide to Common Prescription Sleeping Aids • Orexin Receptor...
Patient Safety Awareness Week
Table of Contents I. Introduction • Medical errors can cause physical, emotional, and financial harm • Effective communication is essential in ensuring patient safety • Patient Safety Awareness Week is a platform to raise awareness and recognize achievements II....
National Women and Girls HIV/AIDS Awareness Day
Table of Contents 1. Introduction: HIV and AIDS in Women 2. Understanding HIV and AIDS 3. How HIV Drugs Work 4. The Role We Can Play to Prevent HIV 5. Ways to Help End HIV A. Learn the Facts About HIV and AIDS B. Use Condoms During Sex C. Limit Your Number of Sexual...
World Sleep Day
Table of Contents • Introduction to the importance of sleep and World Sleep Day • Sleeping disorders and their impact on health • Obstructive Sleep Apnea, its symptoms and treatment options • Insomnia, its types, causes and treatment options • Periodic Limb Movement...
Trisomy awareness month
Table of Contents • Introduction: What is Trisomy? • Some of the Indications of Trisomy disorders during pregnancy • Trisomy Awareness Month: Purpose and Ways to get involved • Trisomy 21 - Down Syndrome: Types, Symptoms and Features • Trisomy 18 - Edwards Syndrome:...
World Kidney Day
Table of Contents: • Introduction • World Kidney Day and Its Objectives • What is Chronic Kidney Disease? • Indications of Chronic Kidney Disease • Early Detection • Treatment of Chronic Kidney Disease Globally an estimated 850 million people are suffering from...
Poison prevention – Attention for accidents
Table of Contents • Accidental Poisonings as a Public Health Problem • Caustic Poisons Act of 1927 • Factors Contributing to Pediatric Poisoning • Unintentional Overdoses in Children Age 6 to 12 Years • Suicidal Poisonings and Adolescent Suicide • Factors Contributing...
Multiple Sclerosis Awareness Month
Table of Contents 1. History of Multiple Sclerosis (MS) 2. Multiple Sclerosis Awareness Month 3. Understanding MS a. MS as an autoimmune inflammatory disease of central nervous system (CNS) b. Mechanisms of MS at the molecular level c. Pathophysiological mechanisms of...
Kidney Health Month
Table of Contents 1. Functions of Kidneys 2. Kidney Disease and its Risk Factors 3. National Kidney Month and Free Screenings 4. Anatomy and Physiology of Kidneys 5. Roles of Kidneys in the Body 6. Causes and Types of Kidney Diseases 7. Acute Kidney Injury (AKI) 8....
National Nutrition Month
Table of Contents 1. Importance of Adequate and Proper Nutrition 2. Nutrition Education and Counseling 3. Occupational Influence on Nutritional Awareness 4. Malnutrition and Its Different Forms 5. Social and Environmental Challenges Affecting Nutritional Status 6....
Endometriosis Awareness Month
Table of Contents I. Introduction A. Definition and epidemiology of endometriosis B. Importance of Endometriosis Awareness Month II. Pathogenesis and Symptoms of Endometriosis A. Pelvic pain and other symptoms B. Possible explanations for endometriosis III. Diagnosis...
National Colorectal Cancer Awareness Month
Table of Contents 1. Colorectal Cancer: Statistics and Risk Factors 2. Screening Recommendations for Colorectal Cancer 3. Limitations of Current Screening Methods 4. Need for Non-Invasive and Reliable Diagnostics for Colorectal Cancer 5. Molecular Pathways of...