P4HA2 Lysate – Tissue Lysate

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

P4HA2: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.2; Oxidoreductase; Secreted, signal peptide; Amino Acid Metabolism - arginine and proline; Secreted

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum lumen; endoplasmic reticulum; cytoplasm; nucleus

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen-proline 4-dioxygenase activity; electron carrier activity; L-ascorbic acid binding; iron ion binding

Biological Process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline