PRKAG1 Lysate – Tissue Lysate

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.

Protein type: Protein kinase, regulatory subunit; Autophagy

Chromosomal Location of Human Ortholog: 12q12-q14

Cellular Component: membrane; nucleus; cytosol; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; ADP binding; protein kinase binding; ATP binding; protein kinase activity; AMP binding

Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; positive regulation of protein kinase activity; regulation of glycolysis; insulin receptor signaling pathway; spermatogenesis; cell cycle arrest; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process