NGF/NGF Beta (NGF) ELISA Kit for Monkey

Principle of the Assay: 215 Monkey primate NGF/NGF Beta ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate NGF/NGF Beta has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: S122-A241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate NGF/NGF Beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate NGF/NGF Beta amount of sample captured in plate.

Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male monkey primate submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is monkey primate 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Small volumes of NGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1751044 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NGF/NGF Beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGF. The ELISA analytical biochemical technique of the MBS1751044 kit is based on NGF antibody-NGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).