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BIRC4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6164746

The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BIRC4, Monoclonal Antibody
Full Product Name
BIRC4 (X-Linked Inhibitor of Apoptosis, API3, BIRC4, ILP1, MIHA, XLP2) (AP)
Product Gene Name
Product Synonym Names
X-Linked Inhibitor of Apoptosis; API3; BIRC4; ILP1; MIHA; XLP2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1F4
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes BIRC4.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
56,685 Da
Immunogen
BIRC4 (AAH32729, 51aa-150aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
GFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDIS
Conjugate
AP

Product Categories/Family for anti-BIRC4 antibody

Applications Tested/Suitable for anti-BIRC4 antibody
Western Blot (WB), In situ Proximity Ligation Assay (Cell)
Application Notes for anti-BIRC4 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-BIRC4 antibody

Proximity Ligation Analysis of protein-protein interactions between AKT1 and XIAP HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-BIRC4 Monoclonal Antibody – Cat# MBS6164746

Testing Data of anti-BIRC4 antibody

Detection limit for recombinant GST tagged XIAP is 0.03 ng/ml as a capture antibody.
Mouse Anti-BIRC4 Monoclonal Antibody, Testing Data – Cat# MBS6164746

Immunofluorescence (IF) of anti-BIRC4 antibody

Immunofluorescence of monoclonal antibody to XIAP on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-BIRC4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6164746

Immunofluorescence (IF) of anti-BIRC4 antibody

Immunofluorescence of monoclonal antibody to XIAP on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-BIRC4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6164746

Other Notes

Small volumes of anti-BIRC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BIRC4 purchase

MBS6164746 is an antibody targeting BIRC4. Also known as BIRC4 (X-Linked Inhibitor of Apoptosis, API3, BIRC4, ILP1, MIHA, XLP2) (AP). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BIRC4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
X-linked inhibitor of apoptosis
NCBI Official Synonym Full Names
X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
NCBI Official Symbol
XIAP
NCBI Official Synonym Symbols
API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
NCBI Protein Information
E3 ubiquitin-protein ligase XIAP

NCBI Summary for BIRC4

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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