Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488 (MBS212208A488)
Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
Staining of KG1 cells with Mouse anti Human CD105 (MBS212208)
Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647 (MBS212208A647)
Staining of KG1 cells with Mouse anti Human CD105RPE
Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.
Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
Staining of KG1 cells with Mouse anti Human CD105:FITC (MBS210726)
Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
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