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CD150 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213767

Rat anti Mouse CD150 antibody, clone 9D1 recognizes murine CD150, also known as signalling lymphocyte activation molecule (SLAM). Murine CD150 is a 75kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and has been shown to be upregulated… More details

$605 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD150, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD150:RPE
Product Gene Name
anti-CD150 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9D1
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
36,590 Da
3D Structure
ModBase 3D Structure for Q9QUM4
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
CHO cells stably transfected with mouse CD150
Fusion Partners||Spleen cells from immunised rats were fused with cells of a myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD150 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD150 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD150 antibody

Staining of mouse thymus with Rat anti Mouse CD150 (MBS212638)
Rat Anti-CD150 Monoclonal Antibody – Cat# MBS213767

Testing Data of anti-CD150 antibody

Staining of mouse thymus cells with Rat anti Mouse CD150:Alexa Fluor 647 (MBS212637A647)
Rat Anti-CD150 Monoclonal Antibody, Testing Data – Cat# MBS213767

Testing Data of anti-CD150 antibody

Staining of mouse thymus cells with Rat anti Mouse CD150: Alexa Fluor 488 (MBS212637A488)
Rat Anti-CD150 Monoclonal Antibody, Testing Data – Cat# MBS213767

Testing Data of anti-CD150 antibody

Staining of mouse thymus cells with Rat anti Mouse CD150 (MBS212637)
Rat Anti-CD150 Monoclonal Antibody, Testing Data – Cat# MBS213767

Testing Data of anti-CD150 antibody

Staining of mouse thymus with Rat anti Mouse CD150: Azide Free (MBS210236)
Rat Anti-CD150 Monoclonal Antibody, Testing Data – Cat# MBS213767

Testing Data of anti-CD150 antibody

Staining of mouse thymus with Rat anti Mouse CD150: RPE
Rat Anti-CD150 Monoclonal Antibody, Testing Data – Cat# MBS213767

Other Notes

Small volumes of anti-CD150 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD150 purchase

MBS213767 is an antibody targeting CD150. Also known as RAT ANTI MOUSE CD150:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD150. It may not necessarily be applicable to this product.

NCBI GI #
29336066
NCBI GeneID
27218
NCBI Accession #
NP_038758.2
UniProt Primary Accession #
Q9QUM4
UniProt Secondary Accession #
Q9QXZ3
UniProt Related Accession #
Q9QUM4
GenBank Nucleotide #
NM_013730.4
NCBI Official Full Name
signaling lymphocytic activation molecule
NCBI Official Synonym Full Names
signaling lymphocytic activation molecule family member 1
NCBI Official Symbol
Slamf1
NCBI Official Synonym Symbols
Slam; CD150; IPO-3; CDw150; ESTM51; AA177906; 4933415F16
UniProt Protein Name
Signaling lymphocytic activation molecule
UniProt Synonym Protein Names
CD_antigen: CD150
Protein Family
Signaling lymphocytic activation molecule
UniProt Gene Name
Slamf1
UniProt Synonym Gene Names
Slam
UniProt Entry Name
SLAF1_MOUSE
NCBI Protein Information
signaling lymphocytic activation molecule; signaling lymphocyte activation molecule

UniProt Comments for CD150

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: regulation of catalytic activity; regulation of vesicle fusion; lymphocyte activation
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Research Articles on CD150

1. The goal of the current study was to determine whether Slam haplotype affected NKT and Vgamma4+ T-cell responses subsequent to coxsackievirus b3 infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD150 antibody

 Products by Pathway  Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with anti-CD150 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with CD150 and Inflammation
 Necrosis Antibodies  >4 publications with CD150 and Necrosis
 Kidney Diseases Antibodies  >1 publications with CD150 and Kidney Diseases
 Disease Models, Animal Antibodies  >1 publications with CD150 and Disease Models, Animal
 Fetal Growth Retardation Antibodies  >1 publications with CD150 and Fetal Growth Retardation
 Heart Diseases Antibodies  >1 publications with CD150 and Heart Diseases

Organs/Tissues associated with anti-CD150 antibody

 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >6 publications with CD150 and Thymus
 Bone Antibodies  >5 publications with CD150 and Bone
 Bone Marrow Antibodies  >5 publications with CD150 and Bone Marrow
 Spleen Antibodies  >3 publications with CD150 and Spleen
 Embryonic Tissue Antibodies  >1 publications with CD150 and Embryonic Tissue
 Lymph Node Antibodies  >1 publications with CD150 and Lymph Node
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