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CFLAR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6177994

MBS6177994 is an antibody targeting CFLAR. Also known as CFLAR (CASP8 and FADD-like Apoptosis Regulator, CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS) (FITC). Host: Mouse. Mouse monoclonal… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CFLAR, Monoclonal Antibody
Full Product Name
CFLAR (CASP8 and FADD-like Apoptosis Regulator, CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS) (FITC)
Product Gene Name
Product Synonym Names
CASP8 and FADD-like Apoptosis Regulator; CASH; CASP8AP1; CLARP; Casper; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1G1
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified
Specificity
Recognizes CFLAR.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CFLAR (AAH01602, 178aa-277aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
AGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYE
Conjugate
FITC

Product Categories/Family for anti-CFLAR antibody

Applications Tested/Suitable for anti-CFLAR antibody
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CFLAR antibody
Applications are based on unconjugated antibody.

Immunoprecipitation (IP) of anti-CFLAR antibody

Immunoprecipitation of CFLAR transfected lysate using anti-CFLAR monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CFLAR MaxPab rabbit polyclonal antibody.
Mouse Anti-CFLAR Monoclonal Antibody – Cat# MBS6177994

Western Blot (WB) of anti-CFLAR antibody

Western Blot analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M03), clone 1G1.

Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-CFLAR Monoclonal Antibody, Western Blot (WB) – Cat# MBS6177994

Immunofluorescence (IF) of anti-CFLAR antibody

Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 30 ug/ml]
Mouse Anti-CFLAR Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6177994

Immunofluorescence (IF) of anti-CFLAR antibody

Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 30 ug/ml]
Mouse Anti-CFLAR Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6177994

Other Notes

Small volumes of anti-CFLAR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CFLAR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
CASP8 and FADD-like apoptosis regulator
NCBI Official Synonym Full Names
CASP8 and FADD like apoptosis regulator
NCBI Official Symbol
CFLAR
NCBI Official Synonym Symbols
CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
NCBI Protein Information
CASP8 and FADD-like apoptosis regulator

NCBI Summary for CFLAR

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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