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CRISPR/CAS9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375451

MBS375451 is an antibody targeting CRISPR/Cas9. Also known as CRISPR/Cas9 Monoclonal Antibody [7A9-3A3]-BSA/Azide Free. Reactive in Streptococcus pyogene. Host: Mouse. Mouse Anti-CRISPR/Cas9 [7A9-3A3] More details

$895 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CRISPR/Cas9, Monoclonal Antibody
Full Product Name
CRISPR/Cas9 Monoclonal Antibody [7A9-3A3]-BSA/Azide Free
Product Gene Name
anti-CRISPR/Cas9 antibody
Product Synonym Names
Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
7A9-3A3
Host
Mouse
Species Reactivity
Streptococcus pyogene
Form/Format
Purified antibody in PBS. Does not contain sodium azide or BSA
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Molecular Weight
158,441 Da
3D Structure
ModBase 3D Structure for Q99zw2
Immunogen
This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9
Target Antigen
CRISPR/Cas9
Myeloma/Fusion Partners
X63.Ag8-653
Pubmed ID
33398349, 33441761, 33767386, 34035409, 34321211, 34430300, 32550896, 32661087, 32986839, 32602833, 31964705, 32267915, 31980609, 31900423, 31051099, 30805613, 31361218, 31410472, 30858603, 30617347, 31184714, 31006810, 30073181, 30082871, 30291237, 30213867, 29519936, 29759820, 30537986, 29927077, 27820801, 27486969, 28694259, 28624187, 29141659, 28851710, 27499201, 27638686, 26245833
Applications Tested/Suitable for anti-CRISPR/Cas9 antibody
Immunoblot (IB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CRISPR/Cas9 antibody
IB: 0.1-1ug/ml

Western Blot (WB) of anti-CRISPR/Cas9 antibody

Western blot-Anti CRISPR/Cas9 Antibody [7A9-3A3]
Mouse Anti-CRISPR/CAS9 Monoclonal Antibody – Cat# MBS375451

Immunofluorescence (IF) of anti-CRISPR/Cas9 antibody

IF-CRISPR/Cas9 Monoclonal Antibody [7A9-3A3] on HeLa cells
Mouse Anti-CRISPR/CAS9 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS375451

Other Notes

Small volumes of anti-CRISPR/Cas9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CRISPR/Cas9. It may not necessarily be applicable to this product.

NCBI GI #
15675041
NCBI GeneID
901176
NCBI Accession #
NP_269215.1
UniProt Primary Accession #
Q99zw2
GenBank Nucleotide #
NC_002737.2
NCBI Official Full Name
conserved hypothetical protein
NCBI Official Symbol
SPy_1046
UniProt Protein Name
CRISPR-associated endonuclease Cas9/Csn1
UniProt Synonym Protein Names
SpyCas9
UniProt Gene Name
cas9
UniProt Entry Name
CAS9_STRP1
NCBI Protein Information
hypothetical protein

UniProt Comments for CRISPR/Cas9

Cas9: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA probably requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (By similarity). Cas9/crRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Binding to dsDNA target requires a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif). When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming

Protein type: EC 3.1.-.-
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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