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HDAC3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6166222

MBS6166222 is an antibody targeting HDAC3. Also known as HDAC3 (Histone Deacetylase 3, HD3, RPD3, RPD3-2) (APC). Host: Mouse. Mouse monoclonal antibody raised against a full length recombinant HDAC3. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HDAC3, Monoclonal Antibody
Full Product Name
HDAC3 (Histone Deacetylase 3, HD3, RPD3, RPD3-2) (APC)
Product Gene Name
Product Synonym Names
Histone Deacetylase 3; HD3; RPD3; RPD3-2
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2F9-4B7
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes HDAC3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
HDAC3 (AAH00614, 1aa-428aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAKTVAYFYDPDVGNFHYGAGHPMKPHRLALTHSLVLHYGLYKKMIVFKPYQASQHDMCRFHSEDYIDFLQRVSPTNMQGFTKSLNAFNVGDDCPVFPGLFEFCSRYTGASLQGATQLNNKICDIAINWAGGLHHAKKFEASGFCYVNDIVIGILELLKYHPRVLYIDIDIHHGDGVQEAFYLTDRVMTVSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKHLFQPVINQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVKSFNIPLLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI
Conjugate
APC

Product Categories/Family for anti-HDAC3 antibody

Applications Tested/Suitable for anti-HDAC3 antibody
Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-HDAC3 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-HDAC3 antibody

Detection limit for recombinant GST tagged HDAC3 is 1 ng/ml as a capture antibody.
Mouse Anti-HDAC3 Monoclonal Antibody – Cat# MBS6166222

Immunoprecipitation (IP) of anti-HDAC3 antibody

Immunoprecipitation of HDAC3 transfected lysate using anti-HDAC3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HDAC3 MaxPab rabbit polyclonal antibody.
Mouse Anti-HDAC3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6166222

Immunofluorescence (IF) of anti-HDAC3 antibody

Immunofluorescence of monoclonal antibody to HDAC3 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-HDAC3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166222

Immunofluorescence (IF) of anti-HDAC3 antibody

Immunofluorescence of monoclonal antibody to HDAC3 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-HDAC3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166222

Other Notes

Small volumes of anti-HDAC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HDAC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Histone deacetylase 3
NCBI Official Synonym Full Names
histone deacetylase 3
NCBI Official Symbol
HDAC3
NCBI Official Synonym Symbols
HD3; RPD3; KDAC3; RPD3-2
Protein Family
NCBI Protein Information
histone deacetylase 3

NCBI Summary for HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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