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CD15 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9700387

The product of FUT4 transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
CD15, Monoclonal Antibody
Full Product Name
CD15 Monoclonal Antibody
Product Gene Name
anti-CD15 antibody
Product Synonym Names
Mouse Anti-CD15 Monoclonal Antibody; FUT4; ELFT; FCT3A; Alpha-(1,3)-fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
Purity/Purification
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
The antibody detects endogenous CD15 protein.
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
OMIM
M58596 mRNA
3D Structure
ModBase 3D Structure for P22083
Immunogen
Synthetic Peptide
Applications Tested/Suitable for anti-CD15 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
Application Notes for anti-CD15 antibody
IF: 1:100-1:200
IHC-P: 1:100

Immunohistochemistry (IHC) of anti-CD15 antibody

Fig.1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, CD15 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Mouse Anti-CD15 Monoclonal Antibody – Cat# MBS9700387

Immunofluorescence (IF) of anti-CD15 antibody

Fig.2. Immunofluorescence analysis of human liver cancer tissue. 1, CD15 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Mouse Anti-CD15 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9700387

Immunohistochemistry (IHC) of anti-CD15 antibody

Fig.3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using CD 15 Mouse mAb diluted at 1:500.
Mouse Anti-CD15 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9700387

Other Notes

Small volumes of anti-CD15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD15 purchase

MBS9700387 is an antibody targeting CD15. Also known as CD15 Monoclonal Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD15. It may not necessarily be applicable to this product.

NCBI GI #
4503811
NCBI GeneID
2526
NCBI Accession #
NP_002024.1
UniProt Primary Accession #
P22083
UniProt Related Accession #
P22083
GenBank Nucleotide #
NP_002024.1
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
UniProt Synonym Protein Names
ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
Protein Family
CD151 antigen
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4

NCBI Summary for CD15

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]

UniProt Comments for CD15

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Research Articles on CD15

1. these results suggest that uPA/uPAR induces FUT4 expression, and trophoblast cell invasion mediated by AP1 transcription factor (c-Fos and c-Jun).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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