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CD33 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6141056

CD33 is a 67kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD33, Monoclonal Antibody
Full Product Name
CD33 (Myeloid Cell Surface Antigen CD33, Sialic Acid-binding Ig-like Lectin 3, Siglec-3, gp67, SIGLEC3) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D12-G4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CD33.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,293 Da
Immunogen
Full length recombinant corresponding to aa1-364 from human CD33 (AAH28152.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
Conjugate
Biotin

Product Categories/Family for anti-CD33 antibody

Applications Tested/Suitable for anti-CD33 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CD33 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD33 antibody

Western Blot detection against Immunogen (65.78kD).
Mouse Anti-CD33 Monoclonal Antibody – Cat# MBS6141056

Western Blot (WB) of anti-CD33 antibody

CD33 monoclonal antibody, Western Blot analysis of CD33 expression in HL-60.
Mouse Anti-CD33 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141056

Western Blot (WB) of anti-CD33 antibody

Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody. Lane 1: CD33 transfected lysate (39.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-CD33 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141056

Western Blot (WB) of anti-CD33 antibody

Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-CD33 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141056

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD33 purchase

MBS6141056 is an antibody targeting CD33. Also known as CD33 (Myeloid Cell Surface Antigen CD33, Sialic Acid-binding Ig-like Lectin 3, Siglec-3, gp67, SIGLEC3) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens CD33 molecule, mRNA
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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