Mouse Anti-CD50 Monoclonal Antibody – Cat# MBS666269

CD50 Antibody – Mouse Monoclonal

Name: CD50 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666269
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666269

Information: Human CD50 is an adhesion molecule that interacts with LFA-1 (CD18/CD11a) and provides a costimulatory signal for T cells which is thought to be essential for initiation of immune responses. Antibody 186-2G9 recognizes the D1 domain of the ICAM-3… More details

$435 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD50, Monoclonal Antibody

Full Product Name

anti-human CD50-FITC

Product Gene Name

anti-CD50 antibody

Product Synonym Names

Monoclonal anti-human CD50 (ICAM-3)/FITC

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

186-2G9

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

Volume: 0.2ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Five x 105 cultured Jurkat-4G cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD50/FITC at a dilution factor of 1:50 (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.11 log10 fluorescent units when compared to a Mouse IgG2b/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD50 antibody.

Conjugation

FITC

Production

Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 10.1.

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Stimulated human leukocytes

Applications Tested/Suitable for anti-CD50 antibody

Flow Cytometry (FC/FACS), PS

Application Notes for anti-CD50 antibody

1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD50 antibody

1) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579. 2) C.L. Holness, et al, (1995) J Biol Chem 270: 877-884. 3) Y. van Kooyk, et al, (1996) J Exp Med 183: 1247-1252. 4) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.

Other Notes

Small volumes of anti-CD50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD50 purchase

MBS666269 is an antibody targeting CD50. Also known as anti-human CD50-FITC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD50. It may not necessarily be applicable to this product.

Protein Family

Intercellular adhesion molecule

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.