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CD60 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666099

Information: Human CD60 antigen is a glycolipid determinant found on about 30% of peripheral blood T cells. The glycolipid determinant is linked to a glycoprotein which can transduce activation signals. Antibody UM4D4 recognizes a 9-0-acetylated disialosyl… More details

$435 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD60, Monoclonal Antibody
Full Product Name
anti-human CD60-Purified Preservative Free
Product Gene Name
anti-CD60 antibody
Product Synonym Names
Monoclonal anti-human CD60
Clonality
Monoclonal
Isotype
IgM
Clone Number
UM4D4
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not Freeze! Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured Molt-4 human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD60 at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.
Production
Antibody from (low FBS containing) tissue culture supernatant was purified to 95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin) using size exclusion chromatography. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human rheumatoid synovial T cell line (ST-1)
Applications Tested/Suitable for anti-CD60 antibody
Flow Cytometry (FC/FACS), FX, Western Blot (WB)

Testing Data of anti-CD60 antibody

Mouse Anti-CD60 Monoclonal Antibody – Cat# MBS666099

Product References and Citations for anti-CD60 antibody

J.B. Higgs, et al, (1988) J Immunol 140: 3758-3765. D.A. Fox, et al, (1990) Cell Immunol 128: 480-489. B. Kniep, et al, (1993) Blood 82: 1776-1786. E.P. Rieber & G. Rank, (1994) J Exp Med 179: 1385-1390. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 398-404.

Other Notes

Small volumes of anti-CD60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD60 purchase

MBS666099 is an antibody targeting CD60. Also known as anti-human CD60-Purified Preservative Free. Reactive in Human. Host: Mouse.
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