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CD68 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380749

MBS4380749 is an antibody targeting CD68. Also known as CD68 (Macrophage Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD68, Monoclonal Antibody
Full Product Name
CD68 (Macrophage Marker)
Product Gene Name
anti-CD68 antibody
Product Synonym Names
GP110; LAMP4; Microsialin; Macrosialin; SCARD1; Scavenger Receptor Class D Member-1
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
C68/2709
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
110kDa
3D Structure
ModBase 3D Structure for P34810
Immunogen
Recombinant fragment of human CD68 protein (around aa 150-221) (exact sequence is proprietary)
Positive Control
Tonsil, Lymph Node or Spleen
Cellular Localization
Cytoplasmic
Chromosome Location
17p13.1
Applications Tested/Suitable for anti-CD68 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistology (Formalin-Fixed)
Application Notes for anti-CD68 antibody
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-CD68 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (C68/2709).
Mouse Anti-CD68 Monoclonal Antibody – Cat# MBS4380749

Testing Data of anti-CD68 antibody

Mouse Anti-CD68 Monoclonal Antibody, Testing Data – Cat# MBS4380749

Product References and Citations for anti-CD68 antibody

Holness, C.L. and Simmons, D.L. 1993. Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81: 1607-1613.

Other Notes

Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.

NCBI GI #
91199550
NCBI GeneID
968
NCBI Accession #
NP_001035148.1
UniProt Primary Accession #
P34810
UniProt Secondary Accession #
Q53HR6; Q53XI3; Q96BI7; B4DVT4
UniProt Related Accession #
P34810
GenBank Nucleotide #
NM_001040059.1
NCBI Official Full Name
macrosialin isoform B
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
CD68
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
Gp110; CD_antigen: CD68
Protein Family
Macrosialin
UniProt Gene Name
CD68
NCBI Protein Information
macrosialin

NCBI Summary for CD68

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD68

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.CautionCD68 is a commonly used marker for macrophages. However, a number of studies (PubMed:15194571, PubMed:15647451 and PubMed:18405323) have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.
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Research Articles on CD68

1. Combined detection of tumor-associated macrophages markers, CD68 and Sema4D, in gastric carcinoma tissue shows potential to predict the trend of gastric carcinoma progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD68 antibody

 Reference Product  PubMed Publications
 CD163 antibody  >792 publications with CD68 and CD163
 CD34 antibody  >747 publications with CD68 and CD34
 TNF antibody  >740 publications with CD68 and TNF
 CCL2 antibody  >354 publications with CD68 and CCL2
 IL6 antibody  >90 publications with CD68 and IL6
 PTPRC antibody  >41 publications with CD68 and PTPRC
 ITGAM antibody  >23 publications with CD68 and ITGAM
 VIM antibody  >15 publications with CD68 and VIM
 ITGAX antibody  >4 publications with CD68 and ITGAX

Pathways associated with anti-CD68 antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram

Diseases associated with anti-CD68 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2555 publications with CD68 and Inflammation
 Necrosis Antibodies  >1207 publications with CD68 and Necrosis
 Disease Models, Animal Antibodies  >843 publications with CD68 and Disease Models, Animal
 Fibrosis Antibodies  >713 publications with CD68 and Fibrosis
 Lung Diseases Antibodies  >568 publications with CD68 and Lung Diseases
 Atherosclerosis Antibodies  >504 publications with CD68 and Atherosclerosis
 Kidney Diseases Antibodies  >503 publications with CD68 and Kidney Diseases
 Liver Diseases Antibodies  >478 publications with CD68 and Liver Diseases
 Heart Diseases Antibodies  >396 publications with CD68 and Heart Diseases
 Hyperplasia Antibodies  >279 publications with CD68 and Hyperplasia

Organs/Tissues associated with anti-CD68 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3723 publications with CD68 and Blood
 Vascular Antibodies  >1826 publications with CD68 and Vascular
 Muscle Antibodies  >1400 publications with CD68 and Muscle
 Brain Antibodies  >1171 publications with CD68 and Brain
 Bone Antibodies  >1162 publications with CD68 and Bone
 Skin Antibodies  >1142 publications with CD68 and Skin
 Liver Antibodies  >812 publications with CD68 and Liver
 Lymph Node Antibodies  >683 publications with CD68 and Lymph Node
 Kidney Antibodies  >674 publications with CD68 and Kidney
 Lung Antibodies  >673 publications with CD68 and Lung
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