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CDH5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6247312

MBS6247312 is an antibody targeting CDH5. Also known as CDH5 (Cadherin-5, 7B4 Antigen, Vascular Endothelial Cadherin, VE-cadherin, CD144) (MaxLight 750). Reactive in Human. Host: Mouse. More details

$1065 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CDH5, Monoclonal Antibody
Full Product Name
CDH5 (Cadherin-5, 7B4 Antigen, Vascular Endothelial Cadherin, VE-cadherin, CD144) (MaxLight 750)
Product Gene Name
anti-CDH5 antibody
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
13A168
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human VE-Cadherin.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Molecular Weight
91.5kDa (810aa) 70-100kDa (SDS-PAGE under reducing conditions.)
OMIM
601120
3D Structure
ModBase 3D Structure for P33151
Immunogen
HUVEC cells
Conjugate
MaxLight750

Product Categories/Family for anti-CDH5 antibody

Antibodies; Cadherin
Applications Tested/Suitable for anti-CDH5 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

Immunocytochemistry (ICC) of anti-CDH5 antibody

Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HUVEC cells using VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Mouse Anti-CDH5 Monoclonal Antibody – Cat# MBS6247312

Western Blot (WB) of anti-CDH5 antibody

Western Blotting Analysis: Representative lot data. HUVEC cell lysate was probed with VE-cadherin (1:2000 dilution). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates VE-cadherin (~120kD).
Mouse Anti-CDH5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6247312

Product References and Citations for anti-CDH5 antibody

1. Prigozhina, N.L., et al. (2007). Assay Drug Dev Technol. 5(1):29-48. 2. Cohen, C.D., et al. (2006). Proc Natl Acad Sci USA. 103(15):5682-5687. 3. Wary, K.K., et al. (2003). Mol Cancer. 2(1):25. 4. Humtsoe, J.O., et al. (2003). EMBO J. 22(7): 1539-1554. 5. Herren, B., et al. (1998). Mol Biol Cell. 9(6): 1589-1601. 6. Harraz, M., et al. (2001). Stem Cells. 19:304- 312. 7. Yang, S., et al. (1999). American Journal of Pathology. 155:887-895. 8. Reyes, M., et al. (2002). J Clin Invest. 109:337-346. 9. Matsumoto, Y., et al. (1993). Transplantation. 56(1):69-75. 10. Horak, E.R., et al. (1992). Lancet. 340(8828): 1120-1124. 11. Gerecht-Nir, S., et al. (2003). Lab Invest. 83(12):1811-1820. 12. Konstantoulaki, M., et al. (2003). AmJ Physiol Lung Cell Mol Physiol. 285:L434-L442. 13. Martin-Padura, I., et al. (1995) J. Pathol. 175(1):51-57. 14. Lampugnani, M.G., et al. (1992). J. Cell Biol. 118:1511-1522. 15. Tang, D.G., et al. (1993). J. Biol. Chem. 268(30):22883-22894. 16. Brevario, F., et al. (1995). Aterioschler Thromb Vasc Biol. 15(8):1229-1239.

Other Notes

Small volumes of anti-CDH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH5. It may not necessarily be applicable to this product.

NCBI GI #
166362713
NCBI GeneID
1003
NCBI Accession #
NP_001786
UniProt Primary Accession #
P33151
UniProt Secondary Accession #
Q4VAI5; Q4VAI6
UniProt Related Accession #
P33151
GenBank Nucleotide #
NM_001795.4
NCBI Official Full Name
cadherin-5 preproprotein
NCBI Official Synonym Full Names
cadherin 5
NCBI Official Symbol
CDH5
NCBI Official Synonym Symbols
7B4; CD144
UniProt Protein Name
Cadherin-5
UniProt Synonym Protein Names
7B4 antigen; Vascular endothelial cadherin
Protein Family
Cadherin
UniProt Gene Name
CDH5
UniProt Synonym Gene Names
VE-cadherin
UniProt Entry Name
CADH5_HUMAN
NCBI Protein Information
cadherin-5

NCBI Summary for CDH5

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]
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UniProt Comments for CDH5

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation
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Research Articles on CDH5

1. This study demonstrated that changes in gene expression of CDH5 and CLDN5 due to shear stress within individual differentiations also revealed no trend.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDH5 antibody

 Reference Product  PubMed Publications
 KDR antibody  >4 publications with CDH5 and KDR
 VIM antibody  >2 publications with CDH5 and VIM
 DSP antibody  >1 publications with CDH5 and DSP
 CTNNB1 antibody  >1 publications with CDH5 and CTNNB1
 CTNNA1 antibody  >1 publications with CDH5 and CTNNA1

Pathways associated with anti-CDH5 antibody

 Products by Pathway  Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Fluid Shear Stress And Atherosclerosis Pathway antibodies  Fluid Shear Stress And Atherosclerosis Pathway Diagram
 Fluid Shear Stress And Atherosclerosis Pathway antibodies  Fluid Shear Stress And Atherosclerosis Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-CDH5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >10 publications with CDH5 and Neoplasms
 Cardiovascular Diseases Antibodies  >6 publications with CDH5 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >3 publications with CDH5 and Breast Neoplasms
 Myocardial Infarction Antibodies  >2 publications with CDH5 and Myocardial Infarction
 Hemorrhage Antibodies  >2 publications with CDH5 and Hemorrhage
 Necrosis Antibodies  >2 publications with CDH5 and Necrosis
 Inflammation Antibodies  >2 publications with CDH5 and Inflammation
 Neovascularization, Pathologic Antibodies  >2 publications with CDH5 and Neovascularization, Pathologic
 Brain Diseases Antibodies  >2 publications with CDH5 and Brain Diseases
 Heart Diseases Antibodies  >2 publications with CDH5 and Heart Diseases

Organs/Tissues associated with anti-CDH5 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >21 publications with CDH5 and Vascular
 Embryonic Tissue Antibodies  >10 publications with CDH5 and Embryonic Tissue
 Brain Antibodies  >6 publications with CDH5 and Brain
 Heart Antibodies  >4 publications with CDH5 and Heart
 Kidney Antibodies  >4 publications with CDH5 and Kidney
 Placenta Antibodies  >4 publications with CDH5 and Placenta
 Lung Antibodies  >3 publications with CDH5 and Lung
 Muscle Antibodies  >3 publications with CDH5 and Muscle
 Umbilical Cord Antibodies  >3 publications with CDH5 and Umbilical Cord
 Bone Antibodies  >3 publications with CDH5 and Bone
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