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DARS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6136165

MBS6136165 is an antibody targeting DARS. Also known as DARS (Aspartyl-tRNA Synthetase, Cytoplasmic, Aspartate-tRNA Ligase, AspRS, Cell Proliferation-inducing Gene 40 Protein, PIG40) APC. Reactive in Human. Host: Mouse. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DARS, Monoclonal Antibody
Full Product Name
DARS (Aspartyl-tRNA Synthetase, Cytoplasmic, Aspartate-tRNA Ligase, AspRS, Cell Proliferation-inducing Gene 40 Protein, PIG40) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DARS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
59.3 kDa (521aa)
OMIM
603084
3D Structure
ModBase 3D Structure for P14868
Immunogen
Partial recombinant corresponding to aa393-500 from human DARS (NP_001340) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLT
Conjugate
APC

Product Categories/Family for anti-DARS antibody

Applications Tested/Suitable for anti-DARS antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-DARS antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DARS antibody

Western Blot detection against Immunogen (37.62kD).
Mouse Anti-DARS Monoclonal Antibody – Cat# MBS6136165

Western Blot (WB) of anti-DARS antibody

Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody. Lane 1: DARS transfected lysate (57.1kD). Lane 2: Non-transfected lysate.
Mouse Anti-DARS Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136165

Immunohistochemistry (IHC) of anti-DARS antibody

Immunoperoxidase of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].
Mouse Anti-DARS Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6136165

Testing Data of anti-DARS antibody

Detection limit for recombinant GST tagged DARS is ~0.3ng/ml as a capture antibody.
Mouse Anti-DARS Monoclonal Antibody, Testing Data – Cat# MBS6136165

Western Blot (WB) of anti-DARS antibody

Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-DARS Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136165

Western Blot (WB) of anti-DARS antibody

DARS monoclonal antibody Western Blot analysis of DARS expression in HeLa.
Mouse Anti-DARS Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136165

Product References and Citations for anti-DARS antibody

1. Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome? Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L.Neoplasia. 2009 Nov;11(11):1194-207.

Other Notes

Small volumes of anti-DARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DARS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
aspartate--tRNA ligase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase
NCBI Official Symbol
DARS
NCBI Official Synonym Symbols
HBSL; aspRS
UniProt Protein Name
Aspartate--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names
Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein
UniProt Gene Name
DARS
UniProt Synonym Gene Names
AspRS
UniProt Entry Name
SYDC_HUMAN
NCBI Protein Information
aspartate--tRNA ligase, cytoplasmic

NCBI Summary for DARS

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
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UniProt Comments for DARS

DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Translation; EC 6.1.1.12; Ligase

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity

Biological Process: tRNA aminoacylation for protein translation; translation; aspartyl-tRNA aminoacylation; protein complex assembly; gene expression

Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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