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DOT1L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6152201

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DOT1L, Monoclonal Antibody
Full Product Name
DOT1L (KMT4, Histone-lysine N-methyltransferase, H3 Lysine-79 Specific, Histone H3-K79 Methyltransferase, H3-K79-HMTase, DOT1-like Protein, Lysine N-methyltransferase 4, KIAA1814, DKFZp586P1823) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6A6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DOT1L.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
165kDa
OMIM
AF509504 mRNA
3D Structure
ModBase 3D Structure for Q8TEK3
Immunogen
Partial recombinant corresponding to aa3-109 from human DOT1L (NP_115871) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLR
Conjugate
HRP
Applications Tested/Suitable for anti-DOT1L antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-DOT1L antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DOT1L antibody

Western Blot detection against Immunogen (37.77kD).
Mouse Anti-DOT1L Monoclonal Antibody – Cat# MBS6152201

Immunofluorescence (IF) of anti-DOT1L antibody

Immunofluorescence of monoclonal antibody to DOT1L on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-DOT1L Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6152201

Testing Data of anti-DOT1L antibody

Detection limit for recombinant GST tagged DOT1L is ~0.03ng/ml as a capture antibody.
Mouse Anti-DOT1L Monoclonal Antibody, Testing Data – Cat# MBS6152201

Other Notes

Small volumes of anti-DOT1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOT1L purchase

MBS6152201 is an antibody targeting DOT1L. Also known as DOT1L (KMT4, Histone-lysine N-methyltransferase, H3 Lysine-79 Specific, Histone H3-K79 Methyltransferase, H3-K79-HMTase, DOT1-like Protein, Lysine N-methyltransferase 4, KIAA1814, DKFZp586P1823) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DOT1L. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase, H3 lysine-79 specific
NCBI Official Synonym Full Names
DOT1 like histone lysine methyltransferase
NCBI Official Symbol
DOT1L
NCBI Official Synonym Symbols
DOT1; KMT4
UniProt Protein Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Synonym Protein Names
DOT1-like protein; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4
UniProt Gene Name
DOT1L
UniProt Synonym Gene Names
KIAA1814; KMT4; H3-K79-HMTase
NCBI Protein Information
histone-lysine N-methyltransferase, H3 lysine-79 specific

NCBI Summary for DOT1L

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOT1L antibody

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