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FCGR1A / CD64 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249232

MBS249232 is an antibody targeting FCGR1A / CD64. Also known as Anti-FCGR1A / CD64 Antibody (clone 3D3) IHC-plus. Reactive in Human. Host: Mouse. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FCGR1A / CD64, Monoclonal Antibody
Full Product Name
Anti-FCGR1A / CD64 Antibody (clone 3D3) IHC-plus
Product Gene Name
anti-FCGR1A / CD64 antibody
Product Synonym Names
Mouse Monoclonal [clone 3D3] (IgG1) to Human FCGR1A / CD64; Human FCGR1A / CD64; CD64A; CD64 antigen; FCG1; FcgammaRIa; IGFR1; IgG Fc receptor I; Fc-gamma receptor I A1; Fc-gamma RIA; FCGR1; Fc gamma receptor; Fc-gamma RI; FCRI
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3D3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human CD64
Preparation and Storage
Store at -20°C. Avoid freeze-thaw cycles. Stable for 12 months from date of receipt if properly stored.
OMIM
146760
3D Structure
ModBase 3D Structure for P12314
Immunogen Species
FCGR1A / CD64 antibody was raised against Human
Immunogen
FCGR1A / CD64 antibody was raised against full length human recombinant protein of human FCGR1A(NP_000557) produced in HEK293T cell.
Antigen Type
Recombinant protein
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-FCGR1A / CD64 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-FCGR1A / CD64 antibody
IHC-Paraffin (10 ug/ml)
Immunofluorescence (1:100)
Western Blot (1:4000)
(optimal dilution to be determined by the researcher)

Other Notes

Small volumes of anti-FCGR1A / CD64 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR1A / CD64. It may not necessarily be applicable to this product.

NCBI GI #
10835133
NCBI GeneID
2209
NCBI Accession #
NP_000557.1
UniProt Primary Accession #
P12314
UniProt Secondary Accession #
P12315; Q5QNW7; Q92495; Q92663
UniProt Related Accession #
P12314
GenBank Nucleotide #
NM_000566.3
NCBI Official Full Name
high affinity immunoglobulin gamma Fc receptor I
NCBI Official Synonym Full Names
Fc fragment of IgG receptor Ia
NCBI Official Symbol
FCGR1A
NCBI Official Synonym Symbols
CD64; FCRI; CD64A; IGFR1
UniProt Protein Name
High affinity immunoglobulin gamma Fc receptor I
UniProt Synonym Protein Names
Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD_antigen: CD64
UniProt Gene Name
FCGR1A
UniProt Synonym Gene Names
FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
UniProt Entry Name
FCGR1_HUMAN
NCBI Protein Information
high affinity immunoglobulin gamma Fc receptor I

NCBI Summary for FCGR1A / CD64

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

UniProt Comments for FCGR1A / CD64

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: early endosome membrane; integral to membrane; plasma membrane

Molecular Function: IgG binding; protein binding; receptor signaling protein activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; immune response; innate immune response; phagocytosis, engulfment; regulation of immune response; signal transduction
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Research Articles on FCGR1A / CD64

1. we address of monocytes functional status through assessment of the patterns of expression of Fcgamma receptors CD64, CD32, CD16 and CD180 receptor on monocytes from CLL patients and healthy individuals using specific mAbs and flow cytometry.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FCGR1A / CD64 antibody

 Reference Product  PubMed Publications
 FCGR3A antibody  >11 publications with FCGR1A / CD64 and FCGR3A
 SYK antibody  >10 publications with FCGR1A / CD64 and SYK
 CRP antibody  >8 publications with FCGR1A / CD64 and CRP
 LAT antibody  >1 publications with FCGR1A / CD64 and LAT
 HCK antibody  >1 publications with FCGR1A / CD64 and HCK

Pathways associated with anti-FCGR1A / CD64 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cross-presentation Of Soluble Exogenous Antigens (endosomes) Pathway antibodies  Cross-presentation Of Soluble Exogenous Antigens (endosomes) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-FCGR1A / CD64 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >33 publications with FCGR1A / CD64 and Inflammation
 Necrosis Antibodies  >16 publications with FCGR1A / CD64 and Necrosis
 Cardiovascular Diseases Antibodies  >10 publications with FCGR1A / CD64 and Cardiovascular Diseases
 Respiratory Tract Diseases Antibodies  >10 publications with FCGR1A / CD64 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >8 publications with FCGR1A / CD64 and Lung Diseases
 Hematologic Diseases Antibodies  >5 publications with FCGR1A / CD64 and Hematologic Diseases
 Asthma Antibodies  >4 publications with FCGR1A / CD64 and Asthma
 Atherosclerosis Antibodies  >3 publications with FCGR1A / CD64 and Atherosclerosis
 Kidney Diseases Antibodies  >3 publications with FCGR1A / CD64 and Kidney Diseases
 Respiratory Hypersensitivity Antibodies  >3 publications with FCGR1A / CD64 and Respiratory Hypersensitivity

Organs/Tissues associated with anti-FCGR1A / CD64 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >87 publications with FCGR1A / CD64 and Blood
 Lung Antibodies  >6 publications with FCGR1A / CD64 and Lung
 Vascular Antibodies  >5 publications with FCGR1A / CD64 and Vascular
 Kidney Antibodies  >3 publications with FCGR1A / CD64 and Kidney
 Intestine Antibodies  >2 publications with FCGR1A / CD64 and Intestine
 Brain Antibodies  >2 publications with FCGR1A / CD64 and Brain
 Nerve Antibodies  >1 publications with FCGR1A / CD64 and Nerve
 Stomach Antibodies  >1 publications with FCGR1A / CD64 and Stomach
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