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FGG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147202

MBS6147202 is an antibody targeting FGG. Also known as FGG (Fibrinogen gamma chain, PRO2061, FGG) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FGG, Monoclonal Antibody
Full Product Name
FGG (Fibrinogen gamma chain, PRO2061, FGG) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1F2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human FGG.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,497 Da
Immunogen
Partial recombinant protein corresponding to aa31-130 from human FGG (AAH07044) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RDNCCILDERFGSYCPTTCGIADFLSTYQTKVDKDLQSLEDILHQVENKTSEVKQLIKAIQLTYNPDESSKPNMIDAATLKSRKMLEEIMKYEASILTHD
Conjugate
FITC

Product Categories/Family for anti-FGG antibody

Applications Tested/Suitable for anti-FGG antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-FGG antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-FGG antibody

Western Blot detection against immunogen (36.63kD).
Mouse Anti-FGG Monoclonal Antibody – Cat# MBS6147202

Western Blot (WB) of anti-FGG antibody

Western Blot analysis of FGG expression in Hela NE using 126795.
Mouse Anti-FGG Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147202

Immunohistochemistry (IHC) of anti-FGG antibody

Immunoperoxidase to FGG on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue using 126795 (1ug/ml).
Mouse Anti-FGG Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6147202

Testing Data of anti-FGG antibody

Detection limit for 126795 ~0.3ng/ml as a capture antibody.
Mouse Anti-FGG Monoclonal Antibody, Testing Data – Cat# MBS6147202

Testing Data of anti-FGG antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ICAM1 and FGG. HeLa cells were stained with ICAM1 rabbit purified polyclonal (1:1200) and 126795 (1:50). Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex
Mouse Anti-FGG Monoclonal Antibody, Testing Data – Cat# MBS6147202

Product References and Citations for anti-FGG antibody

1. Identification of Novel Diagnostic Biomarkers for Asthma and Chronic Obstructive Pulmonary Disease. Verrills NM, Irwin JA, He XY, Wood LG, Powell H, Simpson JL, McDonald VM, Sim A, Gibson PG.Am J Respir Crit Care Med. 2011 Mar 18. 2. Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics. Horlock C, Shakib F, Mahdavi J, Jones NS, Sewell HF, Ghaemmaghami AM.Genome Biol. 2007;8(3):R30.

Other Notes

Small volumes of anti-FGG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FGG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens fibrinogen gamma chain, mRNA
NCBI Official Synonym Full Names
fibrinogen gamma chain
NCBI Official Symbol
FGG
Protein Family
NCBI Protein Information
fibrinogen gamma chain; fibrinogen, gamma polypeptide

NCBI Summary for FGG

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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