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FLIP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200126

FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FLIP, Monoclonal Antibody
Full Product Name
FLIP antibody
Product Gene Name
Product Synonym Names
CASP8 and FADD-like apoptosis regulator isoform 1; CFLAR; CASH; CASP8AP1; CLARP; MRIT; Caspase homolog; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; Cellular FLICE-like inhibitory protein; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT8B12
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
44,112 Da
OMIM
AF010127 mRNA
3D Structure
ModBase 3D Structure for O15519
Antigen Species
Human
Immunogen
Recombinant human FLIP (1-480aa) purified from E. coli
Clone Note
Anti-human FLIP mAb, clone AT8B12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FLIP protein.

Product Categories/Family for anti-FLIP antibody

Applications Tested/Suitable for anti-FLIP antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-FLIP antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: K562 cell lysate
Mouse Anti-FLIP Monoclonal Antibody – Cat# MBS200126

Immunofluorescence (IF) of anti-FLIP antibody

ICC/IF analysis of FLIP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FLIP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Mouse Anti-FLIP Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200126

Product References and Citations for anti-FLIP antibody

Yu JW, Shi Y., (2008) Oncogene. 27 (48):6216-27; Du C, et al., (2005) Kidney Int. 67 (4):1397-409

Other Notes

Small volumes of anti-FLIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FLIP purchase

MBS200126 is an antibody targeting FLIP. Also known as FLIP antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FLIP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CASP8 and FADD-like apoptosis regulator isoform 1
NCBI Official Synonym Full Names
CASP8 and FADD like apoptosis regulator
NCBI Official Symbol
CFLAR
NCBI Official Synonym Symbols
CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
UniProt Protein Name
CASP8 and FADD-like apoptosis regulator
UniProt Synonym Protein Names
Caspase homolog; CASH
UniProt Gene Name
CFLAR
UniProt Synonym Gene Names
CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
NCBI Protein Information
CASP8 and FADD-like apoptosis regulator

NCBI Summary for FLIP

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
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UniProt Comments for FLIP

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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