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FYN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6141989

Fyn is a member of the Src family of protein tyrosine kinases. Fyn has been shown to be involved in T cell and B cell activation as well as keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FYN, Monoclonal Antibody
Full Product Name
FYN (Tyrosine-protein Kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like Kinase, SLK, p59-Fyn) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G11-F9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human FYN.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
54,513 Da
Immunogen
Full length recombinant corresponding to aa1-482 from human FYN (AAH32496) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL
Conjugate
Biotin

Product Categories/Family for anti-FYN antibody

Applications Tested/Suitable for anti-FYN antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-FYN antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-FYN antibody

Western Blot detection against Immunogen (78.76kD).
Mouse Anti-FYN Monoclonal Antibody – Cat# MBS6141989

Western Blot (WB) of anti-FYN antibody

Western Blot analysis of FYN expression in transfected 293T cell line by FYN monoclonal antibody. Lane 1: FYN transfected lysate (55.43kD). Lane 2: Non-transfected lysate.
Mouse Anti-FYN Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141989

Testing Data of anti-FYN antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCD and FYN. HeLa cells were stained with PRKCD rabbit purified polyclonal 1:1200 and FYN mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-FYN Monoclonal Antibody, Testing Data – Cat# MBS6141989

Other Notes

Small volumes of anti-FYN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FYN purchase

MBS6141989 is an antibody targeting FYN. Also known as FYN (Tyrosine-protein Kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like Kinase, SLK, p59-Fyn) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FYN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens FYN oncogene related to SRC, FGR, YES, mRNA
NCBI Official Synonym Full Names
FYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
FYN
NCBI Official Synonym Symbols
SLK; SYN; p59-FYN
NCBI Protein Information
tyrosine-protein kinase Fyn; FYN oncogene related to SRC, FGR, YES; OKT3-induced calcium influx regulator; c-syn protooncogene; proto-oncogene Syn; proto-oncogene c-Fyn; src-like kinase; src/yes-related novel; tyrosine kinase p59fyn(T)

NCBI Summary for FYN

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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