GFAP Antibody – Mouse Monoclonal

Monoclonal

Mouse (BALB/c) IgG2b, kappa

SB61b1

Mouse

Human

Cyanine 3 (CY3) Conjugate

Reacts with human GFAP (~ 50 kDa)1
Glial Fibrillary Acid Protein (GFAP) was discovered by Bignami et al 2 as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.3 Many types of brain tumors, presumably of astrocytic origin, heavily express GFAP. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells of salivary tumors, and has been reported in erythrocytes. Therefore, antibodies to GFAP are very useful as markers of astrocytic cells and neural stem cells, and for distinguishing neoplasms of astrocytic origin from other neoplasms in the central nervous system.
Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein may also play a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the myelin layer that covers nerve cells. Additionally, GFAP may assist in maintaining the protective blood-brain barrier.
In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. Alexander's disease was recently shown to be caused by point mutations in the protein-coding region of the GFAP gene.4 All forms of Alexander's disease are characterized by the presence of Rosenthal fibers, which are GFAP-containing cytoplasmic inclusions found in astrocytes.

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0; store at 2-8 degree C.
HRP conjugate is supplied in 1.0 mL of stock solution in 50% glycerol/50% phosphate buffered saline, pH.7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.

49,880 Da

chromosome: 17; Location: 17q21

137780

ModBase 3D Structure for P14136

Recombinant human Glial Fibrillary Acid Protein (GFAP)

To ensure acceptable performance, each batch of product is tested by immunohistochemistry and Western blotting to conform to characteristics of a standard reference reagent.