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GPR3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245574

GPR3 has been identified as a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes… More details

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Product Overview

Product Name
GPR3, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3B4-G3] (IgG2a,k) to Human GPR3
Product Gene Name
anti-GPR3 antibody
Product Synonym Names
Anti-GPR3 Antibody (clone 3B4-G3) IHC-plus; GPR3; ACCA; ACCA orphan receptor; G protein-coupled receptor 3; GPCR3; Hacca; G-protein coupled receptor 3; Human GPR3
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3B4-G3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
35,010 Da
OMIM
600241
3D Structure
ModBase 3D Structure for P46089
Target Species
Human
Immunogen Description
GPR3 (AAH32702, 1 a.a. ~ 331 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
GPR3 antibody was raised against gPR3 (AAH32702, 1 a.a. ~ 331 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Product Categories/Family for anti-GPR3 antibody

Family: GPCR; Subfamily: Lysophospholipid/Lysosphingolipid
Applications Tested/Suitable for anti-GPR3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), RNA interference
Application Notes for anti-GPR3 antibody
ELISA, IHC-P (5 ug/ml), RNAi, WB
Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. RNAi Knockdown (Antibody validated).

Immunohistochemistry (IHC) of anti-GPR3 antibody

Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-GPR3 Monoclonal Antibody – Cat# MBS245574

Immunohistochemistry (IHC) of anti-GPR3 antibody

Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-GPR3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245574

Western Blot (WB) of anti-GPR3 antibody

Western blot of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody clone 3B4-G3.
Mouse Anti-GPR3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245574

Western Blot (WB) of anti-GPR3 antibody

GPR3 monoclonal antibody, clone 3B4-G3 Western blot of GPR3 expression in Jurkat.
Mouse Anti-GPR3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245574

RNA interference of anti-GPR3 antibody

Western blot of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody, clone 3B4-G3. GAPDH (36.1 kD) used as specificity a.
Mouse Anti-GPR3 Monoclonal Antibody, RNA interference – Cat# MBS245574

ELISA (EIA) of anti-GPR3 antibody

Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-GPR3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245574

Other Notes

Small volumes of anti-GPR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPR3 purchase

MBS245574 is an antibody targeting GPR3. Also known as Mouse Monoclonal [clone 3B4-G3] (IgG2a,k) to Human GPR3. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GPR3. It may not necessarily be applicable to this product.

NCBI GI #
4885325
NCBI GeneID
2827
NCBI Accession #
NP_005272.1
UniProt Primary Accession #
P46089
UniProt Secondary Accession #
A8K570
UniProt Related Accession #
P46089
GenBank Nucleotide #
NM_005281.3
NCBI Official Full Name
G-protein coupled receptor 3
NCBI Official Synonym Full Names
G protein-coupled receptor 3
NCBI Official Symbol
GPR3
NCBI Official Synonym Symbols
ACCA
UniProt Protein Name
G-protein coupled receptor 3
UniProt Synonym Protein Names
ACCA orphan receptor
Protein Family
G-protein coupled receptor
UniProt Gene Name
GPR3
UniProt Synonym Gene Names
ACCA
UniProt Entry Name
GPR3_HUMAN
NCBI Protein Information
G-protein coupled receptor 3; ACCA orphan receptor; adenylate cyclase constitutive activator

NCBI Summary for GPR3

This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]
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UniProt Comments for GPR3

GPR3: Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons and neurite outgrowth. Maintains also meiotic arrest in oocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell cycle regulation; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; regulation of meiosis; positive regulation of cAMP metabolic process; G-protein signaling, adenylate cyclase activating pathway
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Research Articles on GPR3

1. Gpr3 stimulates Abeta production via interactions with APP and beta-arrestin2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPR3 antibody

 Reference Product  PubMed Publications
 GPR4 antibody  >4 publications with GPR3 and GPR4

Pathways associated with anti-GPR3 antibody

 Products by Pathway  Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-GPR3 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >3 publications with GPR3 and Brain Diseases
 Kidney Diseases Antibodies  >1 publications with GPR3 and Kidney Diseases
 Inflammation Antibodies  >1 publications with GPR3 and Inflammation

Organs/Tissues associated with anti-GPR3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >19 publications with GPR3 and Brain
 Muscle Antibodies  >3 publications with GPR3 and Muscle
 Testis Antibodies  >3 publications with GPR3 and Testis
 Eye Antibodies  >2 publications with GPR3 and Eye
 Uterus Antibodies  >1 publications with GPR3 and Uterus
 Bone Antibodies  >1 publications with GPR3 and Bone
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