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GSTA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147486

MBS6147486 is an antibody targeting GSTA1. Also known as GSTA1 (Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1, MGC131939) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GSTA1, Monoclonal Antibody
Full Product Name
GSTA1 (Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1, MGC131939) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2F7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography
Specificity
Recognizes human GSTA1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-222 from human GSTA1 (AAH53578.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF
Conjugate
FITC

Product Categories/Family for anti-GSTA1 antibody

Applications Tested/Suitable for anti-GSTA1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-GSTA1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GSTA1 antibody

Western Blot detection against Immunogen (50.16kD).
Mouse Anti-GSTA1 Monoclonal Antibody – Cat# MBS6147486

Western Blot (WB) of anti-GSTA1 antibody

Western Blot analysis of GSTA1 expression in transfected 293T cell line by GSTA1 monoclonal antibody. Lane 1: GSTA1 transfected lysate (26kD). Lane 2: Non-transfected lysate.
Mouse Anti-GSTA1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147486

Immunohistochemistry (IHC) of anti-GSTA1 antibody

Immunoperoxidase of monoclonal antibody to GSTA1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].
Mouse Anti-GSTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6147486

Testing Data of anti-GSTA1 antibody

Detection limit for recombinant GST tagged GSTA1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-GSTA1 Monoclonal Antibody, Testing Data – Cat# MBS6147486

Product References and Citations for anti-GSTA1 antibody

1. Redox Protein Expression Predicts Radiotherapeutic Response in Early-Stage Invasive Breast Cancer Patients. Woolston CM, Al-Attar A, Storr SJ, Ellis IO, Morgan DA, Martin SG.Int J Radiat Oncol Biol Phys. 2011 Feb 5. 2. Overcoming Glutathione S-Transferase P1-Related Cisplatin Resistance in Osteosarcoma. Pasello M, Michelacci F, Scionti I, Hattinger CM, Zuntini M, Caccuri AM, Scotlandi K, Picci P, Serra M.Cancer Res. 2008 Aug 15;68(16):6661-8.

Other Notes

Small volumes of anti-GSTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GSTA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens glutathione S-transferase alpha 1, mRNA
NCBI Official Synonym Full Names
glutathione S-transferase alpha 1
NCBI Official Symbol
GSTA1
NCBI Official Synonym Symbols
GST2; GTH1; GSTA1-1; GST-epsilon
NCBI Protein Information
glutathione S-transferase A1

NCBI Summary for GSTA1

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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