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IMPDH2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147781

IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IMPDH2, Monoclonal Antibody
Full Product Name
IMPDH2 (Inosine-5'-monophosphate Dehydrogenase 2, IMP Dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II, IMPD2) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E12-B6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human IMPDH2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-514 from human IMPDH2 (AAH12840) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF
Conjugate
FITC

Product Categories/Family for anti-IMPDH2 antibody

Applications Tested/Suitable for anti-IMPDH2 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-IMPDH2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-IMPDH2 antibody

Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody. Lane 1: IMPDH2 transfected lysate (55.8kD). Lane 2: Non-transfected lysate.
Mouse Anti-IMPDH2 Monoclonal Antibody – Cat# MBS6147781

Immunoprecipitation (IP) of anti-IMPDH2 antibody

Immunoprecipitation of IMPDH2 transfected lysate using IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 rabbit polyclonal antibody.
Mouse Anti-IMPDH2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6147781

Testing Data of anti-IMPDH2 antibody

Detection limit for recombinant GST tagged IMPDH2 is ~0.3ng/ml as a capture antibody.
Mouse Anti-IMPDH2 Monoclonal Antibody, Testing Data – Cat# MBS6147781

Western Blot (WB) of anti-IMPDH2 antibody

Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-IMPDH2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147781

Product References and Citations for anti-IMPDH2 antibody

1. Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol. da Costa AN, Mijal RS, Keen JN, Findlay JB, Wild CP.Proteomics. 2011 Mar 9. doi: 10.1002/pmic.201000580.

Other Notes

Small volumes of anti-IMPDH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IMPDH2 purchase

MBS6147781 is an antibody targeting IMPDH2. Also known as IMPDH2 (Inosine-5'-monophosphate Dehydrogenase 2, IMP Dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II, IMPD2) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IMPDH2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens IMP (inosine monophosphate) dehydrogenase 2, mRNA
NCBI Official Synonym Full Names
inosine monophosphate dehydrogenase 2
NCBI Official Symbol
IMPDH2
NCBI Official Synonym Symbols
IMPD2; IMPDH-II
NCBI Protein Information
inosine-5'-monophosphate dehydrogenase 2

NCBI Summary for IMPDH2

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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