Mouse Anti-ITGA5 Monoclonal Antibody – Cat# MBS570237

Figure 1: Flow cytometric analysis of human monocytes showing CD49e positive population

Integrin Alpha 5 (Cd49e) (ITGA5) Antibody – Mouse Monoclonal

Name: ITGA5 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570237
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570237

Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their environment, being both other cells or the extra- cellular matrix. Integrins are a family of heterodimeric membrane… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Integrin alpha 5 (CD49e) (ITGA5), Monoclonal Antibody

Full Product Name

Mouse anti Integrin alpha 5 (CD49e)

Product Gene Name

anti-ITGA5 antibody

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

NKI-SAM-1

Host

Mouse (Balb/c)

Species Reactivity

Human

Form/Format

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity

NKI-SAM-1 recognizes the 135-kDa integrin-alpha5 monocyte-specific antigen. The exact epitope is not known. NKI-SAM-1 strongly reacts with monocytes, and weakly with granulocytes, platelets and T-lymphocytes.

Preparation and Storage

Store at 4 degree C, or in small aliquots at -20 degree C.

Molecular Weight

114,536 Da

Chromosome Location

Chromosome: 12; NC_000012.11 (54789045..54813050, complement). Location: 12q11-q13

OMIM

135620

3D Structure

ModBase 3D Structure for P08648

Source Note

NKI-SAM-1 is a monoclonal antibody obtained by immunization of Balb/c mice with Human monocytes isolated by centrifugal elutriation.

Product Categories/Family for anti-ITGA5 antibody

CD markers; Cell adhesion; Cell adhesion; Immunology

Applications Tested/Suitable for anti-ITGA5 antibody

Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP)

Application Notes for anti-ITGA5 antibody

NKI-SAM-1 is suitable for flow cytometry (using 0.5-1 ug for 106 cells) and can be used to determine the VLA-5 expression in peripheral blood monocytes and monocyte derived dendritic cells B cells and B-cell precursors. NKI-SAM-1 can be applied in immunohistochemistry, immunocytochemistry (frozen sections) and immunoprecipitation. Optimal antibody dilution should be determined by titRation.

Product References and Citations for anti-ITGA5 antibody

Te Velde, A., Klomp, J., Yard, B., De Vries, J. and Figdor, C. (1988). Modulation of phenotypic and functional properties of Human peripheral blood monocytes by IL-4. Journal Immunol 140, 1548-54. Ammon, C., Meyer, S.P., Schwarzfischer, L., Krause, S.W., Andreesen, R. and Kreutz, M. (2000). Comparitive analysis of integrin expression on monocyte-derived macrophages and monocyte-derived dendritic cells. Immunology 100, 364-9. Grassi, F., Dezutter-Dambuyant, C., McIlroy, D., Jacquet, C., Yoneda, K., Imamura, S., Boumsell, L., Schmitt, D., Autran, B., Debre, P., Hosmalin, A. (1998). Monocyte-derived dendritic cells have a phenotype comparable to that of dermal cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol 64, 484-493. Xiao, J., Messinger, Y., Jin, J., Myers, D., Bolen, J. and Uckun, F. (1996). Signal transduction through the b1 integrin Family surface adhesion Molecules VLA-4 and VLA-5 of Human B-cell precursors activates CD19 receptor-associated protein-tyrosine Kinases. J Biol Chem 271, 7659-64. Santoni, G., Birbarelli, P., Hong, L., Gamero, A., Djeu, J. and Piccoli, M. (1995). An a5b1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin. J Med Microbiol 43, 360-7. Klingemann, H.G. and Dedhar, S. (1989). Distribution of integrins on Human peripheral blood mononuclear cells. Blood 74, 1348-54.

Other Notes

Small volumes of anti-ITGA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGA5 purchase

MBS570237 is an antibody targeting ITGA5. Also known as Mouse anti Integrin alpha 5 (CD49e). Reactive in Human. Host: Mouse (Balb/c).

Additional Details

NCBI/Uniprot data below describe general gene information for ITGA5. It may not necessarily be applicable to this product.

NCBI GI #

56237029

NCBI GeneID

3678

NCBI Accession #

NP_002196.2

UniProt Primary Accession #

P08648

UniProt Secondary Accession #

Q96HA5

UniProt Related Accession #

P08648

GenBank Nucleotide #

NM_002205.2

NCBI Official Full Name

integrin alpha-5

NCBI Official Synonym Full Names

integrin, alpha 5 (fibronectin receptor, alpha polypeptide)

NCBI Official Symbol

ITGA5

NCBI Official Synonym Symbols

FNRA; CD49e; VLA5A

UniProt Protein Name

Integrin alpha-5

UniProt Synonym Protein Names

CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5

Protein Family

Integrin

UniProt Gene Name

ITGA5

UniProt Synonym Gene Names

FNRA

UniProt Entry Name

ITA5_HUMAN

NCBI Protein Information

integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit

NCBI Summary for ITGA5

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

UniProt Comments for ITGA5

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; vascular endothelial growth factor receptor 2 binding; metal ion binding; platelet-derived growth factor receptor binding; epidermal growth factor receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; viral reproduction; cell-substrate adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; memory; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; angiogenesis; blood coagulation; cell adhesion; leukocyte migration; wound healing, spreading of epidermal cells; positive regulation of cell migration

Research Articles on ITGA5

1. the identification of ITGA5 -E2/3 splice variant as potential novel adverse prognostic marker for low-risk AML

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGA5 antibody

Reference Product	PubMed Publications
ITGB1 antibody	>7 publications with ITGA5 and ITGB1
ITGB3 antibody	>6 publications with ITGA5 and ITGB3
FN1 antibody	>6 publications with ITGA5 and FN1
ITGB5 antibody	>3 publications with ITGA5 and ITGB5
COL18A1 antibody	>1 publications with ITGA5 and COL18A1
TLN1 antibody	>1 publications with ITGA5 and TLN1

Pathways associated with anti-ITGA5 antibody

Products by Pathway	Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram

Diseases associated with anti-ITGA5 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>15 publications with ITGA5 and Neoplasms
Neoplasm Invasiveness Antibodies	>7 publications with ITGA5 and Neoplasm Invasiveness
Breast Neoplasms Antibodies	>6 publications with ITGA5 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>6 publications with ITGA5 and Neoplasm Metastasis
Carcinoma Antibodies	>5 publications with ITGA5 and Carcinoma
Inflammation Antibodies	>4 publications with ITGA5 and Inflammation
Cardiovascular Diseases Antibodies	>2 publications with ITGA5 and Cardiovascular Diseases
Lung Diseases Antibodies	>2 publications with ITGA5 and Lung Diseases
Nervous System Diseases Antibodies	>2 publications with ITGA5 and Nervous System Diseases
Kidney Diseases Antibodies	>1 publications with ITGA5 and Kidney Diseases

Organs/Tissues associated with anti-ITGA5 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>10 publications with ITGA5 and Vascular
Bone Antibodies	>9 publications with ITGA5 and Bone
Uterus Antibodies	>7 publications with ITGA5 and Uterus
Embryonic Tissue Antibodies	>6 publications with ITGA5 and Embryonic Tissue
Liver Antibodies	>5 publications with ITGA5 and Liver
Bone Marrow Antibodies	>5 publications with ITGA5 and Bone Marrow
Muscle Antibodies	>5 publications with ITGA5 and Muscle
Lung Antibodies	>4 publications with ITGA5 and Lung
Blood Antibodies	>3 publications with ITGA5 and Blood
Connective Tissue Antibodies	>3 publications with ITGA5 and Connective Tissue