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MAGEA3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6153392

MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAGEA3, Monoclonal Antibody
Full Product Name
MAGEA3 (Melanoma-associated Antigen 3, Antigen MZ2-D, Cancer/Testis Antigen 1.3, CT1.3, MAGE-3 Antigen, MAGE3, MGC14613) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6D10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MAGEA3.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
37.1 kDa (337aa) confirmed by MALDI-TOF
OMIM
300174
3D Structure
ModBase 3D Structure for P43357
Immunogen
Partial recombinant corresponding to aa44-114 from human MAGEA3 (NP_005353) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVA
Conjugate
HRP

Product Categories/Family for anti-MAGEA3 antibody

Applications Tested/Suitable for anti-MAGEA3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MAGEA3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAGEA3 antibody

Western Blot detection against Immunogen (33.55kD).
Mouse Anti-MAGEA3 Monoclonal Antibody – Cat# MBS6153392

Testing Data of anti-MAGEA3 antibody

Detection limit for recombinant GST tagged MAGEA3 is ~0.3ng/ml as a capture antibody.
Mouse Anti-MAGEA3 Monoclonal Antibody, Testing Data – Cat# MBS6153392

Other Notes

Small volumes of anti-MAGEA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAGEA3 purchase

MBS6153392 is an antibody targeting MAGEA3. Also known as MAGEA3 (Melanoma-associated Antigen 3, Antigen MZ2-D, Cancer/Testis Antigen 1.3, CT1.3, MAGE-3 Antigen, MAGE3, MGC14613) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGEA3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma-associated antigen 3
NCBI Official Synonym Full Names
MAGE family member A3
NCBI Official Symbol
MAGEA3
NCBI Official Synonym Symbols
HIP8; HYPD; CT1.3; MAGE3; MAGEA6
UniProt Protein Name
Melanoma-associated antigen 3
UniProt Synonym Protein Names
Antigen MZ2-D; Cancer/testis antigen 1.3; CT1.3; MAGE-3 antigen
UniProt Gene Name
MAGEA3
UniProt Synonym Gene Names
MAGE3; CT1.3
UniProt Entry Name
MAGA3_HUMAN
NCBI Protein Information
melanoma-associated antigen 3

NCBI Summary for MAGEA3

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAGEA3

MAGEA3: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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