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MFAP4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249429

MFAP4 Antibody Description: MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell… More details

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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MFAP4, Monoclonal Antibody
Full Product Name
Anti-MFAP4 Antibody (clone AT12D11) IHC-plus
Product Gene Name
anti-MFAP4 antibody
Product Synonym Names
Mouse Monoclonal [clone AT12D11] (IgG1,k) to Human MFAP4; Human MFAP4
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT12D11
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
Protein A purified
Concentration
1 mg/ml (lot specific)
Specificity
Human MFAP4
Preparation and Storage
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
OMIM
600596
3D Structure
ModBase 3D Structure for P55083
Immunogen
MFAP4 antibody was raised against recombinant human MFAP4 (22-255aa) purified from E Coli.
Immunogen Description
Recombinant human MFAP4 (22-255aa) purified from E Coli.
Immunogen Type
Recombinant protein
Target Species
Human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-MFAP4 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
Application Notes for anti-MFAP4 antibody
IHC-P (10 ug/ml)
WB (1:1000)

Immunohistochemistry (IHC) of anti-MFAP4 antibody

Anti-MFAP4 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-MFAP4 Monoclonal Antibody – Cat# MBS249429

Western Blot (WB) of anti-MFAP4 antibody

Western Blot: The extract of lung (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MFAP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-MFAP4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249429

Other Notes

Small volumes of anti-MFAP4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MFAP4 purchase

MBS249429 is an antibody targeting MFAP4. Also known as Anti-MFAP4 Antibody (clone AT12D11) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MFAP4. It may not necessarily be applicable to this product.

NCBI GI #
2506403
NCBI GeneID
4239
NCBI Accession #
P55083.2
UniProt Primary Accession #
P55083
UniProt Secondary Accession #
Q6P680; A8KAJ1; A8MVM2; B4E317
UniProt Related Accession #
P55083
NCBI Official Full Name
Microfibril-associated glycoprotein 4
NCBI Official Synonym Full Names
microfibrillar associated protein 4
NCBI Official Symbol
MFAP4
UniProt Protein Name
Microfibril-associated glycoprotein 4
Protein Family
Microfibril-associated glycoprotein
UniProt Gene Name
MFAP4
UniProt Entry Name
MFAP4_HUMAN
NCBI Protein Information
microfibril-associated glycoprotein 4

NCBI Summary for MFAP4

This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
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UniProt Comments for MFAP4

MFAP4: Could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 is deleted in the Smith-Magenis syndrome (SMS).

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Calcium-binding

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: extracellular matrix; extracellular region; microfibril

Molecular Function: protein binding

Biological Process: cell adhesion; elastic fiber assembly; extracellular matrix organization and biogenesis; fibril organization and biogenesis; UV protection
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Research Articles on MFAP4

1. Serum MFAP4 varies in groups of patients with different cardiovascular conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MFAP4 antibody

 Reference Product  PubMed Publications
 RAI1 antibody  >2 publications with MFAP4 and RAI1
 FBLN5 antibody  >2 publications with MFAP4 and FBLN5

Pathways associated with anti-MFAP4 antibody

 Products by Pathway  Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Molecules Associated With Elastic Fibres Pathway antibodies  Molecules Associated With Elastic Fibres Pathway Diagram

Diseases associated with anti-MFAP4 antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >7 publications with MFAP4 and Lung Diseases
 Liver Diseases Antibodies  >5 publications with MFAP4 and Liver Diseases
 Fibrosis Antibodies  >5 publications with MFAP4 and Fibrosis
 Inflammation Antibodies  >5 publications with MFAP4 and Inflammation
 Disease Models, Animal Antibodies  >2 publications with MFAP4 and Disease Models, Animal
 Hyperplasia Antibodies  >2 publications with MFAP4 and Hyperplasia
 Cardiovascular Abnormalities Antibodies  >2 publications with MFAP4 and Cardiovascular Abnormalities
 Lung Injury Antibodies  >1 publications with MFAP4 and Lung Injury
 Neoplasms, Experimental Antibodies  >1 publications with MFAP4 and Neoplasms, Experimental
 Heart Defects, Congenital Antibodies  >1 publications with MFAP4 and Heart Defects, Congenital

Organs/Tissues associated with anti-MFAP4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >11 publications with MFAP4 and Lung
 Muscle Antibodies  >6 publications with MFAP4 and Muscle
 Liver Antibodies  >6 publications with MFAP4 and Liver
 Heart Antibodies  >5 publications with MFAP4 and Heart
 Skin Antibodies  >4 publications with MFAP4 and Skin
 Connective Tissue Antibodies  >4 publications with MFAP4 and Connective Tissue
 Brain Antibodies  >2 publications with MFAP4 and Brain
 Prostate Antibodies  >1 publications with MFAP4 and Prostate
 Kidney Antibodies  >1 publications with MFAP4 and Kidney
 Uncharacterized Tissue Antibodies  >1 publications with MFAP4 and Uncharacterized Tissue
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