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MPG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6142966

MBS6142966 is an antibody targeting MPG. Also known as MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MPG, Monoclonal Antibody
Full Product Name
MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1E10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MPG.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
30,824 Da
Immunogen
Partial recombinant corresponding to aa1-90 from human MPG (AAH14991) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPA
Conjugate
Biotin

Product Categories/Family for anti-MPG antibody

Applications Tested/Suitable for anti-MPG antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MPG antibody
IF: 40ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MPG antibody

Western Blot detection against Immunogen (35.53kD).
Mouse Anti-MPG Monoclonal Antibody – Cat# MBS6142966

Western Blot (WB) of anti-MPG antibody

MPG monoclonal antibody Western Blot analysis of MPG expression in Hela NE.
Mouse Anti-MPG Monoclonal Antibody, Western Blot (WB) – Cat# MBS6142966

Western Blot (WB) of anti-MPG antibody

Western Blot analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody. Lane 1: MPG transfected lysate (32.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-MPG Monoclonal Antibody, Western Blot (WB) – Cat# MBS6142966

Immunohistochemistry (IHC) of anti-MPG antibody

Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml]
Mouse Anti-MPG Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6142966

Immunofluorescence (IF) of anti-MPG antibody

Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 40ug/ml]
Mouse Anti-MPG Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6142966

Testing Data of anti-MPG antibody

Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.
Mouse Anti-MPG Monoclonal Antibody, Testing Data – Cat# MBS6142966

Product References and Citations for anti-MPG antibody

1. Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair. Chou WC, Wang HC, Wong FH, Ding SL, Wu PE, Shieh SY, Shen CY.EMBO J. 2008 Dec 3;27(23):3140-50. Epub 2008 Oct 30.

Other Notes

Small volumes of anti-MPG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MPG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens N-methylpurine-DNA glycosylase, mRNA
NCBI Official Synonym Full Names
N-methylpurine DNA glycosylase
NCBI Official Symbol
MPG
NCBI Official Synonym Symbols
AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
NCBI Protein Information
DNA-3-methyladenine glycosylase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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