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MSH6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380962

MBS4380962 is an antibody targeting MSH6. Also known as MSH6 (DNA Mismatch Repair Protein). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSH6, Monoclonal Antibody
Full Product Name
MSH6 (DNA Mismatch Repair Protein)
Product Gene Name
Product Synonym Names
DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MSH6/3091
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
163kDa
OMIM
276300
3D Structure
ModBase 3D Structure for P52701
Positive Control
HCT116, MCF-7 cells, Human colon carcinoma (IHC).
Immunogen
Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)
Cellular Localization
Nuclear
Hu-chromosome Location
2p16.3
Applications Tested/Suitable for anti-MSH6 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-MSH6 antibody
ELISA: For coating, order Ab without BSA
IF: 1-2ug/ml
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-MSH6 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Mouse Monoclonal Antibody (MSH6/3091).
Mouse Anti-MSH6 Monoclonal Antibody – Cat# MBS4380962

SDS-Page of anti-MSH6 antibody

SDS-PAGE Analysis Purified MSH6 Mouse Monoclonal Antibody (MSH6/3091). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-MSH6 Monoclonal Antibody, SDS-Page – Cat# MBS4380962

Immunofluorescence (IF) of anti-MSH6 antibody

Immunofluorescence staining of MCF-7 cells using MSH6 Mouse Monoclonal Antibody (MSH6/3091) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Mouse Anti-MSH6 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS4380962

Western Blot (WB) of anti-MSH6 antibody

Western Blot Analysis of HCT116 cell lysate using MSH6 Mouse Monoclonal Antibody (MSH6/3091).
Mouse Anti-MSH6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380962

Testing Data of anti-MSH6 antibody

Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3091) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-MSH6 Monoclonal Antibody, Testing Data – Cat# MBS4380962

Product References and Citations for anti-MSH6 antibody

Peltomaki, P., et al. 1993. Genetic mapping of a locus predisposing to human colorectal cancer. Science 260: 810-812.

Other Notes

Small volumes of anti-MSH6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA mismatch repair protein Msh6 isoform 1
NCBI Official Synonym Full Names
mutS homolog 6
NCBI Official Symbol
MSH6
NCBI Official Synonym Symbols
GTBP; HSAP; p160; GTMBP; HNPCC5
UniProt Protein Name
DNA mismatch repair protein Msh6
UniProt Synonym Protein Names
G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit
UniProt Gene Name
MSH6
UniProt Synonym Gene Names
GTBP; hMSH6; GTBP; GTMBP; p160
UniProt Entry Name
MSH6_HUMAN
NCBI Protein Information
DNA mismatch repair protein Msh6

NCBI Summary for MSH6

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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