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PPARGC1A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206582

PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. This protein can regulate key mitochondrial genes… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PPARGC1A, Monoclonal Antibody
Full Product Name
PPARGC1A antibody
Product Gene Name
anti-PPARGC1A antibody
Product Synonym Names
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha, Ligand effect modulator 6, PPARGC1A, LEM6, PGC1, PGC1A, PPARGC1
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT25C8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
3D Structure
ModBase 3D Structure for Q9UBK2
Immunogen
Recombinant human PPARGC1A (300-540aa) purified from E. coli

Product Categories/Family for anti-PPARGC1A antibody

Epigenomics
Applications Tested/Suitable for anti-PPARGC1A antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-PPARGC1A antibody

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPARGC1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: MCF7 cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: 293T cell lysate
Lane 5.: Mouse brain tissue lysate
Mouse Anti-PPARGC1A Monoclonal Antibody – Cat# MBS206582

Immunofluorescence (IF) of anti-PPARGC1A antibody

ICC/IF analysis of PPARGC1A in HeLa cells. The cell was stained with MBS206582 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-PPARGC1A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS206582

Flow Cytometry (FC) of anti-PPARGC1A antibody

Flow cytometry analysis of PPARGC1A in HeLa cells. The cell was stained with MBS206582 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-PPARGC1A Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS206582

Product References and Citations for anti-PPARGC1A antibody

Lui Z., et al. (2016) Sci Rep. 6: 21382.; Li R., et al. (2016) Med Sci Monit. 22: 3229-3237.

Other Notes

Small volumes of anti-PPARGC1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPARGC1A purchase

MBS206582 is an antibody targeting PPARGC1A. Also known as PPARGC1A antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPARGC1A. It may not necessarily be applicable to this product.

NCBI GI #
7019499
NCBI GeneID
10891
NCBI Accession #
NP_037393
UniProt Primary Accession #
Q9UBK2
UniProt Secondary Accession #
Q3LIG1; Q4W5M7; Q9UN32; B7Z406; G8DM16; I3RTT5; I3RTT6; I3RTT7; I3RTT8; I3RTT9
UniProt Related Accession #
Q9UBK2
GenBank Nucleotide #
NM_013261.4
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha isoform 2
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Synonym Protein Names
Ligand effect modulator 6
Protein Family
Peroxisome proliferator-activated receptor gamma coactivator
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha

NCBI Summary for PPARGC1A

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPARGC1A

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
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Research Articles on PPARGC1A

1. We aimed to explore the potential therapeutic effect of PGC-1alpha by generating a lentiviral vector to express human PGC-1alpha and target it by stereotaxic delivery to hippocampus and cortex of APP23 transgenic mice . Four months after hPGC-1alpha injection, mice showed improved spatial and recognition memory concomitant with a significant reduction in Abeta deposition, associated with a decrease in BACE1 expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPARGC1A antibody

 Reference Product  PubMed Publications
 SIRT1 antibody  >262 publications with PPARGC1A and SIRT1
 TFAM antibody  >191 publications with PPARGC1A and TFAM
 NRF1 antibody  >101 publications with PPARGC1A and NRF1
 SLC2A4 antibody  >63 publications with PPARGC1A and SLC2A4
 PPARG antibody  >56 publications with PPARGC1A and PPARG
 SIRT3 antibody  >45 publications with PPARGC1A and SIRT3
 MTOR antibody  >43 publications with PPARGC1A and MTOR
 PPARA antibody  >32 publications with PPARGC1A and PPARA
 PRKAA2 antibody  >11 publications with PPARGC1A and PRKAA2
 ESRRA antibody  >6 publications with PPARGC1A and ESRRA

Pathways associated with anti-PPARGC1A antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway antibodies  Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Apelin Signaling Pathway antibodies  Apelin Signaling Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram

Diseases associated with anti-PPARGC1A antibody

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >313 publications with PPARGC1A and Insulin Resistance
 Cardiovascular Diseases Antibodies  >258 publications with PPARGC1A and Cardiovascular Diseases
 Inflammation Antibodies  >194 publications with PPARGC1A and Inflammation
 Fatty Liver Antibodies  >180 publications with PPARGC1A and Fatty Liver
 Heart Diseases Antibodies  >158 publications with PPARGC1A and Heart Diseases
 Liver Diseases Antibodies  >156 publications with PPARGC1A and Liver Diseases
 Diabetes Mellitus, Type 2 Antibodies  >155 publications with PPARGC1A and Diabetes Mellitus, Type 2
 Hyperglycemia Antibodies  >102 publications with PPARGC1A and Hyperglycemia
 Necrosis Antibodies  >89 publications with PPARGC1A and Necrosis
 Hypertrophy Antibodies  >89 publications with PPARGC1A and Hypertrophy

Organs/Tissues associated with anti-PPARGC1A antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1130 publications with PPARGC1A and Muscle
 Liver Antibodies  >519 publications with PPARGC1A and Liver
 Heart Antibodies  >315 publications with PPARGC1A and Heart
 Connective Tissue Antibodies  >283 publications with PPARGC1A and Connective Tissue
 Brain Antibodies  >264 publications with PPARGC1A and Brain
 Kidney Antibodies  >96 publications with PPARGC1A and Kidney
 Thyroid Antibodies  >41 publications with PPARGC1A and Thyroid
 Embryonic Tissue Antibodies  >39 publications with PPARGC1A and Embryonic Tissue
 Pancreas Antibodies  >37 publications with PPARGC1A and Pancreas
 Lung Antibodies  >37 publications with PPARGC1A and Lung
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