SVIL Antibody – Mouse Monoclonal

This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Apr 2016]

supervillin: an actin-binding protein that links filamentous actin to the plasma membrane. Binds filamentous actin and myosin II bind within the rsds 1-830 of the amino terminus, an extended intrinsically disordered region (IDR). Forms a high-affinity link between the actin cytoskeleton and the plasma membrane. Four human isoforms are formed by alternative splicing. Isoform 1 (archvillin) is muscle specific and among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Contractile; Actin-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: costamere; focal adhesion; cytoplasm; plasma membrane; podosome; midbody; nucleus; actin cytoskeleton; cleavage furrow

Molecular Function: actin filament binding; protein binding

Biological Process: positive regulation of cytokinesis; skeletal muscle development; cytoskeleton organization and biogenesis