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TDT Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752362

Rabbit IgG Monoclonal antibody for P04053 detection. Tested positive for WB IHC in Human More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TDT, Monoclonal Antibody
Full Product Name
Anti-TDT Monoclonal Antibody
Product Gene Name
anti-TDT antibody
Product Synonym Gene Name
DNTT
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
58,408 Da
3D Structure
ModBase 3D Structure for P04053
Immunogen
A synthesized peptide derived from human TDT Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TDT antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TDT antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-TDT antibody

Figure 1. Western blot analysis of DNTT using anti-DNTT antibody (M05546).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNTT antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNTT.
Rabbit Anti-TDT Monoclonal Antibody – Cat# MBS1752362

Other Notes

Small volumes of anti-TDT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TDT purchase

MBS1752362 is an antibody targeting TDT. Also known as Anti-TDT Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TDT. It may not necessarily be applicable to this product.

NCBI GI #
63054852
NCBI GeneID
1791
NCBI Accession #
NP_001017520.1
UniProt Primary Accession #
P04053
UniProt Secondary Accession #
Q53FH1; Q5W103; Q96E50
UniProt Related Accession #
P04053
GenBank Nucleotide #
NM_001017520.1
NCBI Official Full Name
DNA nucleotidylexotransferase isoform 2
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
NCBI Official Synonym Symbols
TDT
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Synonym Protein Names
Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Protein Family
Tonoplast dicarboxylate transporter
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
TDT1 PublicationManual assertion based on opinion iniRef.10; Terminal transferase
NCBI Protein Information
DNA nucleotidylexotransferase

NCBI Summary for TDT

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for TDT

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
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Research Articles on TDT

1. TdT protein can be found in cells of epithelial origin and specifically sebaceous cells, both benign and malignant.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TDT antibody

 Reference Product  PubMed Publications
 DNTTIP1 antibody  >1 publications with TDT and DNTTIP1

Pathways associated with anti-TDT antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram

Diseases associated with anti-TDT antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with TDT and Neoplasms
 Lymphoma Antibodies  >1 publications with TDT and Lymphoma

Organs/Tissues associated with anti-TDT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with TDT and Blood
 Thymus Antibodies  >2 publications with TDT and Thymus
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