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USP9X Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6013585

MBS6013585 is an antibody targeting USP9X. Also known as USP9X (Ubiquitin-specific Protease 9, X Chromosome, USP9, Probable Ubiquitin Carboxyl-terminal Hydrolase FAF-X, Deubiquitinating Enzyme FAF-X, DFFRX, Fat Facets in Mammals, FAM, hFAM, Fat Facets… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
USP9X, Monoclonal Antibody
Full Product Name
USP9X (Ubiquitin-specific Protease 9, X Chromosome, USP9, Probable Ubiquitin Carboxyl-terminal Hydrolase FAF-X, Deubiquitinating Enzyme FAF-X, DFFRX, Fat Facets in Mammals, FAM, hFAM, Fat Facets Protein-related, X-linked, Ubiquitin Thioesterase FAF-X, Ubi
Product Gene Name
anti-USP9X antibody
Product Synonym Names
Anti -USP9X (Ubiquitin-specific Protease 9, X Chromosome, USP9, Probable Ubiquitin Carboxyl-terminal Hydrolase FAF-X, Deubiquitinating Enzyme FAF-X, DFFRX, Fat Facets in Mammals, FAM, hFAM, Fat Facets Protein-related, X-linked, Ubiquitin Thioesterase FAF-X, Ubi
Immunogen Sequence
MTATTRGSPV GGNDNQGQAP DGQSQPPLQQ NQTSSPDSSN ENSPATPPDE QGQGDAPPQL EDEEPAFPHT DLAKLDDMIN RPRWVVPVLP *
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5D7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human USP9X.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
292,280 Da
Chromosome Location
Chromosome: X; NC_000023.10 (40944727..41095832). Location: Xp11.4
OMIM
300072
3D Structure
ModBase 3D Structure for Q93008
Immunogen
Partial recombinant corresponding to aa1-91 from USP9X (NP_068706) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-USP9X antibody

Antibodies; Abs to Ubiquitin
Applications Tested/Suitable for anti-USP9X antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-USP9X antibody
Suitable for use in ELISA and Western Blot.

Testing Data of anti-USP9X antibody

Detection limit for recombinant GST tagged USP9X is ~0.1ng/ml as a capture antibody.
Mouse Anti-USP9X Monoclonal Antibody – Cat# MBS6013585

Western Blot (WB) of anti-USP9X antibody

USP9X monoclonal antibody Western Blot analysis of USP9X expression in Jurkat
Mouse Anti-USP9X Monoclonal Antibody, Western Blot (WB) – Cat# MBS6013585

Western Blot (WB) of anti-USP9X antibody

USP9X monoclonal antibody Western Blot analysis of USP9X expression in human placenta.
Mouse Anti-USP9X Monoclonal Antibody, Western Blot (WB) – Cat# MBS6013585

Western Blot (WB) of anti-USP9X antibody

Western Blot detection against Immunogen (36.01kD).
Mouse Anti-USP9X Monoclonal Antibody, Western Blot (WB) – Cat# MBS6013585

Other Notes

Small volumes of anti-USP9X antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP9X. It may not necessarily be applicable to this product.

NCBI GI #
39795394
NCBI GeneID
8239
NCBI Accession #
AAH63645.1
UniProt Primary Accession #
Q93008
UniProt Secondary Accession #
O75550; Q8WWT3; Q8WX12
UniProt Related Accession #
Q93008
NCBI Official Full Name
USP9X protein
NCBI Official Synonym Full Names
ubiquitin specific peptidase 9, X-linked
NCBI Official Symbol
USP9X
NCBI Official Synonym Symbols
FAF; FAM; DFFRX
UniProt Protein Name
Probable ubiquitin carboxyl-terminal hydrolase FAF-X
UniProt Synonym Protein Names
Deubiquitinating enzyme FAF-X; Fat facets in mammals; hFAM; Fat facets protein-related, X-linked; Ubiquitin thioesterase FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X
Protein Family
Probable ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name
USP9X
UniProt Synonym Gene Names
DFFRX; FAM; USP9; hFAM
UniProt Entry Name
USP9X_HUMAN
NCBI Protein Information
probable ubiquitin carboxyl-terminal hydrolase FAF-X; hFAM; fat facets in mammals; ubiquitin thioesterase FAF-X; deubiquitinating enzyme FAF-X; ubiquitin thiolesterase FAF-X; fat facets protein related, X-linked; fat facets protein-related, X-linked; Drosophila fat facets related, X-linked; ubiquitin-specific protease 9, X chromosome; ubiquitin-specific processing protease FAF-X; ubiquitin-specific-processing protease FAF-X; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila)

NCBI Summary for USP9X

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for USP9X

USP9X: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin. Widely expressed in embryonic and adult tissues. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: growth cone; membrane; apical part of cell; cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; mitosis; protein deubiquitination; transcription, DNA-dependent; axon extension; in utero embryonic development; hippocampus development; neuron migration; female gamete generation; negative regulation of transcription from RNA polymerase II promoter; cerebellar cortex structural organization; chromosome segregation; post-embryonic development; BMP signaling pathway; cell division; transforming growth factor beta receptor signaling pathway; gene expression

Disease: Mental Retardation, X-linked 99
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Research Articles on USP9X

1. Mcl-1, Bcl-xL and USP9X overexpression are tumor survival mechanisms protective against chemotherapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP9X antibody

 Reference Product  PubMed Publications
 CTNNB1 antibody  >6 publications with USP9X and CTNNB1
 MLLT4 antibody  >5 publications with USP9X and MLLT4
 MCL1 antibody  >4 publications with USP9X and MCL1
 NUAK1 antibody  >3 publications with USP9X and NUAK1
 MARK4 antibody  >3 publications with USP9X and MARK4
 HUWE1 antibody  >2 publications with USP9X and HUWE1
 MTOR antibody  >1 publications with USP9X and MTOR
 ITCH antibody  >1 publications with USP9X and ITCH

Pathways associated with anti-USP9X antibody

 Products by Pathway  Pathway Diagram
 Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway antibodies  Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By TGF-beta Receptor Complex Pathway antibodies  Signaling By TGF-beta Receptor Complex Pathway Diagram
 Transcriptional Activity Of SMAD2/SMAD3:SMAD4 Heterotrimer Pathway antibodies  Transcriptional Activity Of SMAD2/SMAD3:SMAD4 Heterotrimer Pathway Diagram

Diseases associated with anti-USP9X antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >6 publications with USP9X and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with USP9X and Adenocarcinoma
 Brain Diseases Antibodies  >4 publications with USP9X and Brain Diseases
 Nervous System Diseases Antibodies  >4 publications with USP9X and Nervous System Diseases
 Movement Disorders Antibodies  >3 publications with USP9X and Movement Disorders
 Cell Transformation, Neoplastic Antibodies  >3 publications with USP9X and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >3 publications with USP9X and Lung Neoplasms
 Breast Neoplasms Antibodies  >2 publications with USP9X and Breast Neoplasms
 Intestinal Neoplasms Antibodies  >2 publications with USP9X and Intestinal Neoplasms
 Congenital Abnormalities Antibodies  >2 publications with USP9X and Congenital Abnormalities

Organs/Tissues associated with anti-USP9X antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with USP9X and Brain
 Testis Antibodies  >7 publications with USP9X and Testis
 Eye Antibodies  >5 publications with USP9X and Eye
 Ovary Antibodies  >5 publications with USP9X and Ovary
 Blood Antibodies  >4 publications with USP9X and Blood
 Nerve Antibodies  >3 publications with USP9X and Nerve
 Muscle Antibodies  >3 publications with USP9X and Muscle
 Bone Antibodies  >3 publications with USP9X and Bone
 Lung Antibodies  >3 publications with USP9X and Lung
 Pancreas Antibodies  >2 publications with USP9X and Pancreas
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