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VIM Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200459

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the… More details

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Product Overview

Online Datasheet in PDF
Product Name
VIM, Monoclonal Antibody
Full Product Name
VIM antibody
Product Gene Name
anti-VIM antibody
Product Synonym Names
Vimentin; epididymis luminal protein 113; HEL113; CTRCT30
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5H8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
53,652 Da
OMIM
M14144 Genomic DNA
3D Structure
ModBase 3D Structure for P08670
Antigen Species
Human
Immunogen
Recombinant human VIM (1-466aa) purified from E. coli
Clone Note
Anti-human VIM mAb, clone AT5H8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human VIM protein.

Product Categories/Family for anti-VIM antibody

Neuroscience
Applications Tested/Suitable for anti-VIM antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-VIM antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : K562 cell lysate Lane 3 : 293T cell lysate Lane 4 : A549 cell lysate Lane 5 : SK-OV-3 cell lysate Lane 6 : HeLa cell lysate
Mouse Anti-VIM Monoclonal Antibody – Cat# MBS200459

Immunofluorescence (IF) of anti-VIM antibody

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-VIM Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200459

Product References and Citations for anti-VIM antibody

John E. Eriksson.,et al. (2009) J Clin Invest. 119 (7): 1763-1771. ; Cabeen MT.,et al. (2010) Annu Rev Genet. 44: 365-392.

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS200459 is an antibody targeting VIM. Also known as VIM antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.4
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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UniProt Comments for VIM

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

1. Vimentin and its interaction with Shigella flexneri IpaC are dispensable for effector translocation pore formation, but are required for stable docking of Shigella flexneri to cells; moreover, stable docking triggers effector secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VIM antibody

 Reference Product  PubMed Publications
 CASP3 antibody  >8 publications with VIM and CASP3
 AKT1 antibody  >4 publications with VIM and AKT1
 DMD antibody  >1 publications with VIM and DMD
 CASP8 antibody  >1 publications with VIM and CASP8

Pathways associated with anti-VIM antibody

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >199 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >92 publications with VIM and Inflammation
 Disease Models, Animal Antibodies  >57 publications with VIM and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >57 publications with VIM and Neoplasm Metastasis
 Kidney Diseases Antibodies  >49 publications with VIM and Kidney Diseases
 Breast Neoplasms Antibodies  >47 publications with VIM and Breast Neoplasms
 Fibrosis Antibodies  >46 publications with VIM and Fibrosis
 Liver Diseases Antibodies  >43 publications with VIM and Liver Diseases
 Neoplasm Invasiveness Antibodies  >34 publications with VIM and Neoplasm Invasiveness
 Necrosis Antibodies  >33 publications with VIM and Necrosis

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >639 publications with VIM and Brain
 Blood Antibodies  >301 publications with VIM and Blood
 Muscle Antibodies  >142 publications with VIM and Muscle
 Bone Antibodies  >98 publications with VIM and Bone
 Nerve Antibodies  >95 publications with VIM and Nerve
 Lung Antibodies  >81 publications with VIM and Lung
 Kidney Antibodies  >80 publications with VIM and Kidney
 Liver Antibodies  >73 publications with VIM and Liver
 Bone Marrow Antibodies  >60 publications with VIM and Bone Marrow
 Eye Antibodies  >59 publications with VIM and Eye
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