C-Abl (ABL1) Antibody – Mouse Monoclonal

Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in K562 (human chronic myelogenous leukemia) cell line. c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both nucleus and cytoplasm of cells. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to Bcr gene on chromosome 22. The molecular consequence of translocation is generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1. The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains, which is conserved among the Abl family. Phosphorylation of Tyr245 is involved in activation of c-Abl kinase activity (5). Tyrosine 412 is located in the kinase activation loop of c-Abl molecules; phosphorylation of this residue is required for the kinase activity (6). Thr735 is in a conserved 14-3-3 protein binding motif, which can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation of Thr735 in c-Abl may play an important role in regulation of c-Abl localization as well as its function.

Small volumes of anti-ABL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS600727 is an antibody targeting ABL1. Also known as c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl). Reactive in Human, Mouse. Host: Mouse.