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EZH2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6242453

MBS6242453 is an antibody targeting EZH2. Also known as EZH2 (Histone-lysine N-methyltransferase EZH2, Enhancer of Zeste Homolog 2, ENX-1, Lysine N-methyltransferase 6) (APC). Reactive in Human, Mouse. Host: Mouse. More details

$1400 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EZH2, Monoclonal Antibody
Full Product Name
EZH2 (Histone-lysine N-methyltransferase EZH2, Enhancer of Zeste Homolog 2, ENX-1, Lysine N-methyltransferase 6) (APC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
9H236
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human EZH2 (~98kD). Species Crossreactivity: mouse
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Molecular Weight
79,621 Da
OMIM
277590
3D Structure
ModBase 3D Structure for Q15910
Immunogen
Recombinant fusion protein of aa1-286 of human EZH2 tagged with MBP. Cellular Localization: Nucleus. (NP_004447.2)
Conjugate
APC
Applications Tested/Suitable for anti-EZH2 antibody
Western Blot (WB)

Western Blot (WB) of anti-EZH2 antibody

Western Blot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS605447 (1:5000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates EZH2 (~98kD).
Mouse Anti-EZH2 Monoclonal Antibody – Cat# MBS6242453

Product References and Citations for anti-EZH2 antibody

1. Bracken, A.P., et al., Genes Dev. 21(5): 525-530 (2007). 2. Bracken, A.P., et al., Genes Dev. 20: 1123-1136 (2006). 3. Pasini, D., et al., Mol. Cell. Biol. 27: 3769-3779 (2007). 4. Pasini, D., et al., Genes Dev. 22: 1345-1355 (2008).

Other Notes

Small volumes of anti-EZH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EZH2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase EZH2 isoform a
NCBI Official Synonym Full Names
enhancer of zeste 2 polycomb repressive complex 2 subunit
NCBI Official Symbol
EZH2
NCBI Official Synonym Symbols
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
UniProt Protein Name
Histone-lysine N-methyltransferase EZH2
UniProt Synonym Protein Names
ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6
UniProt Gene Name
EZH2
UniProt Synonym Gene Names
KMT6
UniProt Entry Name
EZH2_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase EZH2; enhancer of zeste homolog 2; lysine N-methyltransferase 6

NCBI Summary for EZH2

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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