Rabbit Anti-CDC40 Monoclonal Antibody – Cat# MBS1750101

Western blot analysis of CDC40 expression in (1) HeLa cell lysate; (2) K562 cell lysate (MBS1750101).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC40 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC40

CDC40 Antibody – Rabbit Monoclonal

Name: CDC40 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750101
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750101

Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CDC40, Monoclonal Antibody

Full Product Name

Anti-CDC40 Rabbit Monoclonal Antibody

Product Gene Name

anti-CDC40 antibody

Product Synonym Names

Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17; CDC40; EHB3; PRP17; PRPF17

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

65521 MW

OMIM

AF038392 mRNA

3D Structure

ModBase 3D Structure for O60508

Immunogen

A synthesized peptide derived from human CDC40

Subcellular Localization

Nucleus.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CDC40 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; RNA Processing; splicing; Cell Biology; Cell Cycle; Cell Division; Other Cell Division Antibodies; Cancer

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CDC40 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-CDC40 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Other Notes

Small volumes of anti-CDC40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC40 purchase

MBS1750101 is an antibody targeting CDC40. Also known as Anti-CDC40 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC40. It may not necessarily be applicable to this product.

NCBI GI #

7706657

NCBI GeneID

51362

NCBI Accession #

NP_056975.1

UniProt Primary Accession #

O60508

UniProt Secondary Accession #

O75471; Q5SRN0; Q9UPG1; B2RBC5

UniProt Related Accession #

O60508

GenBank Nucleotide #

NP_056975.1

NCBI Official Full Name

pre-mRNA-processing factor 17

NCBI Official Synonym Full Names

cell division cycle 40

NCBI Official Symbol

CDC40

NCBI Official Synonym Symbols

EHB3; PRP17; PRPF17

UniProt Protein Name

Pre-mRNA-processing factor 17

UniProt Synonym Protein Names

Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17

UniProt Gene Name

CDC40

UniProt Synonym Gene Names

EHB3; PRP17; PRPF17; Ehb3; hPRP17

NCBI Protein Information

pre-mRNA-processing factor 17

NCBI Summary for CDC40

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq, Jul 2008]

Research Articles on CDC40

1. HBx activates NF-kappaB to promote the expression of miR1269b, which augments CDC40 expression, contributing to malignancy in hepatocellular cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC40 antibody

Reference Product	PubMed Publications
DHX38 antibody	>1 publications with CDC40 and DHX38

Pathways associated with anti-CDC40 antibody

Products by Pathway	Pathway Diagram
Cleavage Of Growing Transcript In The Termination Region Pathway antibodies	Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
RNA Polymerase II Transcription Termination Pathway antibodies	RNA Polymerase II Transcription Termination Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome, 35S U5-snRNP Pathway antibodies	Spliceosome, 35S U5-snRNP Pathway Diagram
Transport Of Mature Transcript To Cytoplasm Pathway antibodies	Transport Of Mature Transcript To Cytoplasm Pathway Diagram
Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway antibodies	Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway Diagram

Diseases associated with anti-CDC40 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>1 publications with CDC40 and Kidney Diseases

Organs/Tissues associated with anti-CDC40 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>1 publications with CDC40 and Muscle
Ovary Antibodies	>1 publications with CDC40 and Ovary
Prostate Antibodies	>1 publications with CDC40 and Prostate
Uterus Antibodies	>1 publications with CDC40 and Uterus
Heart Antibodies	>1 publications with CDC40 and Heart
Kidney Antibodies	>1 publications with CDC40 and Kidney
Testis Antibodies	>1 publications with CDC40 and Testis
Brain Antibodies	>1 publications with CDC40 and Brain