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GAPDH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8309740

MBS8309740 is an antibody targeting GAPDH. Reactive in Human, Mouse, Rat. Host: Mouse. Mouse monoclonal antibody to GAPDH More details

$175 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GAPDH, Monoclonal Antibody
Full Product Name
Anti-GAPDH Antibody
Product Gene Name
Product Synonym Names
GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of GAPDH protein.
Preparation and Storage
Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Chromosome Location
Chromosome: 12; NC_000012.11 (6643585..6647537). Location: 12p13
OMIM
138400
3D Structure
ModBase 3D Structure for P04406
Immunogen
Recombinant protein corresponding to GAPDH.
Applications Tested/Suitable for anti-GAPDH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence/Immunocytochemistry (IF/ICC)
Application Notes for anti-GAPDH antibody
WB: 1/3000-1/10000
IHC: 1/50-1/100
IF/ICC: 1/50-1/100

Western Blot (WB) of anti-GAPDH antibody

Western blot analysis of GAPDH expression in Hela (A), mouse brain (B), mouse spleen (C), rat liver (D), rat lung (E) whole cell lysates.
Mouse Anti-GAPDH Monoclonal Antibody – Cat# MBS8309740

Immunohistochemistry (IHC) of anti-GAPDH antibody

Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Mouse Anti-GAPDH Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8309740

Immunofluorescence (IF)/Immunocytochemistry (ICC) of anti-GAPDH antibody

Immunofluorescent analysis of GAPDH staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Mouse Anti-GAPDH Monoclonal Antibody, Immunofluorescence (IF)/Immunocytochemistry – Cat# MBS8309740

Other Notes

Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names
Peptidyl-cysteine S-nitrosylase GAPDH
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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