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ULK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9200567

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and… More details

$240 / 0.08 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ULK3, Monoclonal Antibody
Full Product Name
ULK3 Antibody (C-term)
Product Gene Name
anti-ULK3 antibody
Product Synonym Names
Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3; ULK3
Clonality
Monoclonal
Isotype
IgG1,k
Host
Mouse
Species Reactivity
Human, mouse, rat
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Specificity
Generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
Calculated MW: 53444
OMIM
613472
3D Structure
ModBase 3D Structure for Q6PHR2
Cellular Location
Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence
Tissue Location
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord

Product Categories/Family for anti-ULK3 antibody

Crown Antibodies; Signal Transduction
Applications Tested/Suitable for anti-ULK3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-ULK3 antibody
WB~~1:50
IHC~~1:1000

Immunohistochemistry (IHC) of anti-ULK3 antibody

Immunohistochemical analysis ofparaffin-embedded Human kidney sectionusing Pink1 (MBS9200567). MBS9200567 wasdiluted at 1:50 dilution. A undilutedbiotinylated goat polyvalent antibody wasused as the secondary, followed by DABstaining.
Mouse Anti-ULK3 Monoclonal Antibody – Cat# MBS9200567

Western Blot (WB) of anti-ULK3 antibody

Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). MBS9200567 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Mouse Anti-ULK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS9200567

Product References and Citations for anti-ULK3 antibody

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Daub H.,et al.Mol. Cell 31:438-448(2008).

Other Notes

Small volumes of anti-ULK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ULK3 purchase

MBS9200567 is an antibody targeting ULK3. Also known as ULK3 Antibody (C-term). Reactive in Human, mouse, rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ULK3. It may not necessarily be applicable to this product.

NCBI GI #
656214624
NCBI GeneID
25989
NCBI Accession #
NP_001092906.3
UniProt Primary Accession #
Q6PHR2
UniProt Secondary Accession #
Q9NPN5; Q9UFS4; B2RXK3; B4DFT0; B4DRQ7; D3DW68
UniProt Related Accession #
Q6PHR2
GenBank Nucleotide #
NM_001099436.3
NCBI Official Full Name
serine/threonine-protein kinase ULK3 isoform a
NCBI Official Synonym Full Names
unc-51 like kinase 3
NCBI Official Symbol
ULK3
UniProt Protein Name
Serine/threonine-protein kinase ULK3
UniProt Synonym Protein Names
Unc-51-like kinase 3
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
ULK3
UniProt Entry Name
ULK3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase ULK3

UniProt Comments for ULK3

ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; ULK family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: negative regulation of smoothened signaling pathway; protein amino acid autophosphorylation; autophagy; positive regulation of smoothened signaling pathway; regulation of autophagy; autophagic vacuole formation
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Research Articles on ULK3

1. First named and identified as protein kinase of the ULK family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ULK3 antibody

 Reference Product  PubMed Publications
 GLI1 antibody  >5 publications with ULK3 and GLI1
 GLI2 antibody  >5 publications with ULK3 and GLI2
 MTOR antibody  >2 publications with ULK3 and MTOR

Diseases associated with anti-ULK3 antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >7 publications with ULK3 and Hypertension
 Vascular Diseases Antibodies  >4 publications with ULK3 and Vascular Diseases
 Brain Diseases Antibodies  >2 publications with ULK3 and Brain Diseases
 Atherosclerosis Antibodies  >1 publications with ULK3 and Atherosclerosis
 Mental Disorders Antibodies  >1 publications with ULK3 and Mental Disorders

Organs/Tissues associated with anti-ULK3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with ULK3 and Blood
 Liver Antibodies  >2 publications with ULK3 and Liver
 Heart Antibodies  >2 publications with ULK3 and Heart
 Embryonic Tissue Antibodies  >2 publications with ULK3 and Embryonic Tissue
 Muscle Antibodies  >1 publications with ULK3 and Muscle
 Lung Antibodies  >1 publications with ULK3 and Lung
 Brain Antibodies  >1 publications with ULK3 and Brain
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