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Cytokeratin, Basic (KRT76) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380421

MBS4380421 is an antibody targeting KRT76. Also known as Cytokeratin, Basic (Type II or HMW) (Epithelial Marker). Reactive in Human. Expected to show broad species reactivity.. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin, Basic (KRT76), Monoclonal Antibody
Full Product Name
Cytokeratin, Basic (Type II or HMW) (Epithelial Marker)
Product Gene Name
Product Synonym Names
KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
Clonality
Monoclonal
Isotype
IgG
Clone Number
KRTH/1576R
Host
Rabbit
Species Reactivity
Human. Expected to show broad species reactivity.
Form/Format
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
52-67kDa
3D Structure
ModBase 3D Structure for Q01546
Immunogen
Recombinant full-length human KRT76 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12q13.13
Positive Control
Epithelial cells, Skin or Adenocarcinomas
Applications Tested/Suitable for anti-KRT76 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT76 antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.25-0.5ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-KRT76 antibody

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).
Rabbit Anti-KRT76 Monoclonal Antibody – Cat# MBS4380421

Immunohistochemistry (IHC) of anti-KRT76 antibody

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).
Rabbit Anti-KRT76 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380421

SDS-Page of anti-KRT76 antibody

SDS-PAGE Analysis Purified Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).
Rabbit Anti-KRT76 Monoclonal Antibody, SDS-Page – Cat# MBS4380421

Product References and Citations for anti-KRT76 antibody

Tseng SCG et. al. Cell 1982; 30361. Woodock-Mitchell J et. al. Journal of Cell Biology 1982; 95:580-8.

Other Notes

Small volumes of anti-KRT76 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT76. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 2 oral
NCBI Official Synonym Full Names
keratin 76
NCBI Official Symbol
KRT76
NCBI Official Synonym Symbols
KRT2B; KRT2P; HUMCYT2A
UniProt Protein Name
Keratin, type II cytoskeletal 2 oral
UniProt Synonym Protein Names
Cytokeratin-2P; CK-2P; K2P; Keratin-76; K76; Type-II keratin Kb9
Protein Family
UniProt Gene Name
KRT76
UniProt Synonym Gene Names
KRT2B; KRT2P; CK-2P; K2P; K76
NCBI Protein Information
keratin, type II cytoskeletal 2 oral

NCBI Summary for KRT76

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]

UniProt Comments for KRT76

Probably contributes to terminal cornification.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-KRT76 antibody

 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >1 publications with KRT76 and Mouth
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