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Bad Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632866

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that… More details

$865 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Bad, Monoclonal Antibody
Full Product Name
Bad, phosphorylated (Ser136)
Product Synonym Names
Anti -Bad, phosphorylated (Ser136)
Clonality
Monoclonal
Isotype
IgG
Clone Number
8A6
Host
Rabbit
Species Reactivity
Monkey, Mouse
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Purity/Purification
Supernatant
Supernatant
Specificity
Recognizes transfected levels of mouse Bad protein only when phosphorylated at Ser136. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad (KLH).

Product Categories/Family for anti-Bad antibody

Applications Tested/Suitable for anti-Bad antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Bad antibody
Dilution: Western Blot: 1:500

Western Blot (WB) of anti-Bad antibody

Western Blot analysis of extracts from control 293, GST-Bad transfected 293 and COS cells, untreated or TPA-treated, using MBS632866.
Rabbit Anti-Bad Monoclonal Antibody – Cat# MBS632866

Western Blot (WB) of anti-Bad antibody

Western Blot analysis of immunoprecipitates from COS cells, untreated or treated with TPA, using MBS632866.
Rabbit Anti-Bad Monoclonal Antibody, Western Blot (WB) – Cat# MBS632866

Other Notes

Small volumes of anti-Bad antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bad purchase

MBS632866 is an antibody targeting Bad. Also known as Bad, phosphorylated (Ser136). Reactive in Monkey, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Bad. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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