Rabbit Anti-CCR7 Monoclonal Antibody – Cat# MBS488776

Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR7 antibody 4B12 (MBS488776) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 4B12 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.

CCR7 Antibody – Rabbit Monoclonal

Name: CCR7 Antibody; Monoclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS488776
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488776

Recombinant monoclonal antibody to CCR7. Recombinant Platform with variable regions (i.e. specificity) from The hybridoma 4B12.This antibody was raised by immunizing rats with mouse CCR7 antigen. More details

$635 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCR7, Monoclonal Antibody

Full Product Name

Anti-CCR7 [4B12]

Product Gene Name

anti-CCR7 antibody

Product Synonym Names

EBI1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD197; C-C CKR-7; CC-CKR-7; C-C chemokine receptor type 7

Clonality

Monoclonal

Isotype

Rabbit IgG, kappa

Clone Number

4B12

Host

Rabbit

Species Reactivity

Mouse

Form/Format

PBS with 0.02% Proclin 300.

Purity/Purification

Purified antibody. Protein A affinity purified

Concentration

1mg/ml (lot specific)

Specificity

This antibody is specific to the mouse CCR7 protein, which was confirmed by immunoprecipitation (Ritter et al, 2004).

Preparation and Storage

Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Molecular Weight

42,856 Da

3D Structure

ModBase 3D Structure for P47774

Animal-free Manufacturing Platform

The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.

Chimeric Use Statement

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

PMID

15197229

Product Categories/Family for anti-CCR7 antibody

dendritic cells; DC; T helper cells; CCR7; immuneresponse

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-CCR7 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Functional Assay (FA)

Application Notes for anti-CCR7 antibody

This antibody was used for flow cytometry and the staining quality can be improved by incubation at 37 degree C (Britschgi et al, 2008). This antibody is widely used to monitor the activation of dendritic cells in mice (Ohl et al, 2004; Ritter et al, 2004; Zhao et al, 2019). It was also utilized in immunoprecipitation of the CCR7 receptor protein (Ritter et al, 2004). The antibody does not interfere with ligands binding to the CCR7 receptor, which was confirmed by a transmigration assay (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et al, 2004). The antibody also is not agonistic as it does not activate the receptor (Ritter et al, 2004).

Product References and Citations for anti-CCR7 antibody

Ritter et al. Analysis of the CCR7 expression on murine bone marrowderived and spleen dendritic cells. J Leukoc Biol. 2004 Aug;76(2):472-6. Epub 2004 Jun 14.
Note on publication: The article describes the analysis of CCR7 expression during the maturation of bonemarrow-dendritic cells

Other Notes

Small volumes of anti-CCR7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCR7 purchase

MBS488776 is an antibody targeting CCR7. Also known as Anti-CCR7 [4B12]. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CCR7. It may not necessarily be applicable to this product.

NCBI GI #

678246450

NCBI GeneID

12775

NCBI Accession #

NP_001288642.1

UniProt Primary Accession #

P47774

UniProt Secondary Accession #

Q8CAS2

UniProt Related Accession #

P47774

GenBank Nucleotide #

NM_001301713.1

NCBI Official Full Name

C-C chemokine receptor type 7 isoform b

NCBI Official Synonym Full Names

chemokine (C-C motif) receptor 7

NCBI Official Symbol

Ccr7

NCBI Official Synonym Symbols

EBI1; CCR-7; CD197; Ebi1h; Cdw197; Cmkbr7; CC-CKR-7

UniProt Protein Name

C-C chemokine receptor type 7

UniProt Synonym Protein Names

Epstein-Barr virus-induced G-protein coupled receptor 1; EBI1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD_antigen: CD197

Protein Family

C-C chemokine receptor

UniProt Gene Name

Ccr7

UniProt Synonym Gene Names

Cmkbr7; Ebi1; Ebi1h; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1

UniProt Entry Name

CCR7_MOUSE

NCBI Protein Information

C-C chemokine receptor type 7

UniProt Comments for CCR7

CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR

Cellular Component: cell surface; external side of plasma membrane; integral to membrane; intracellular; membrane; plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; G-protein coupled receptor activity; protein binding; signal transducer activity

Biological Process: actin cytoskeleton organization and biogenesis; activation of JNK activity; chemotaxis; establishment of T cell polarity; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells; immune response; inflammatory response; lymph node development; lymphocyte chemotaxis across high endothelial venule; myeloid dendritic cell chemotaxis; negative thymic T cell selection; positive regulation of dendritic cell antigen processing and presentation; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of humoral immune response; positive regulation of hypersensitivity; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-12 production; positive regulation of phagocytosis; positive regulation of receptor-mediated endocytosis; positive regulation of T cell proliferation; positive regulation of T cell receptor signaling pathway; positive regulation of T-helper 1 cell differentiation; positive regulation of tumor necrosis factor production; regulation of interferon-gamma production; regulation of interleukin-1 beta secretion; regulation of tolerance induction to self antigen; response to lipopolysaccharide; signal transduction

Research Articles on CCR7

1. mucosal draining lymph nodes express CCR7, which has a role in trafficking of RORgamma innate lymphoid cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCR7 antibody

Reference Product	PubMed Publications
Ccl19 antibody	>482 publications with CCR7 and Ccl19
Cxcl12 antibody	>168 publications with CCR7 and Cxcl12
Cxcl13 antibody	>56 publications with CCR7 and Cxcl13
Ccl17 antibody	>28 publications with CCR7 and Ccl17
TCA4 antibody	>8 publications with CCR7 and TCA4

Pathways associated with anti-CCR7 antibody

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-CCR7 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>421 publications with CCR7 and Inflammation
Necrosis Antibodies	>250 publications with CCR7 and Necrosis
Disease Models, Animal Antibodies	>158 publications with CCR7 and Disease Models, Animal
Lung Diseases Antibodies	>140 publications with CCR7 and Lung Diseases
Hypersensitivity Antibodies	>113 publications with CCR7 and Hypersensitivity
Liver Diseases Antibodies	>79 publications with CCR7 and Liver Diseases
Neoplasms, Experimental Antibodies	>64 publications with CCR7 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>52 publications with CCR7 and Breast Neoplasms
Fibrosis Antibodies	>47 publications with CCR7 and Fibrosis
Atherosclerosis Antibodies	>46 publications with CCR7 and Atherosclerosis

Organs/Tissues associated with anti-CCR7 antibody

Organ/Tissue Name	Pubmed Publications
Lymph Node Antibodies	>832 publications with CCR7 and Lymph Node
Bone Marrow Antibodies	>270 publications with CCR7 and Bone Marrow
Skin Antibodies	>253 publications with CCR7 and Skin
Spleen Antibodies	>183 publications with CCR7 and Spleen
Liver Antibodies	>128 publications with CCR7 and Liver
Thymus Antibodies	>114 publications with CCR7 and Thymus
Embryonic Tissue Antibodies	>34 publications with CCR7 and Embryonic Tissue
Mammary Gland Antibodies	>2 publications with CCR7 and Mammary Gland
Uncharacterized Tissue Antibodies	>1 publications with CCR7 and Uncharacterized Tissue