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F4/80 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2558003

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of… More details

$130 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F4/80, Monoclonal Antibody
Full Product Name
Anti-Mouse F4/80 Monoclonal Antibody (PE Conjugated) [CI:A3-1]
Product Gene Name
Product Synonym Gene Name
Mouse F4/80
Product Synonym Names
Adhesion G protein-coupled receptor E1; Adgre1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Adgre1; Emr1; Gpf480
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CI:A3-1
Host
Rat
Species Reactivity
Mouse
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.5 mg/mL (lot specific)
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.
Matching Pairs
3D Structure
ModBase 3D Structure for Q61549
Conjugation
PE
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-F4/80 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-F4/80 antibody
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Flow Cytometry (FC/FACS) of anti-F4/80 antibody

Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(PE Conjugated)(filled gray curve). Unstained macrophages (blank black curve) are used as control.
Rat Anti-F4/80 Monoclonal Antibody – Cat# MBS2558003

Other Notes

Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F4/80 purchase

MBS2558003 is an antibody targeting F4/80. Also known as Anti-Mouse F4/80 Monoclonal Antibody (PE Conjugated) [CI:A3-1]. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Adhesion G protein-coupled receptor E1
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E1
NCBI Official Symbol
Adgre1
NCBI Official Synonym Symbols
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 1
UniProt Synonym Protein Names
Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
UniProt Gene Name
Emr1
UniProt Synonym Gene Names
Gpf480
UniProt Entry Name
EMR1_MOUSE
NCBI Protein Information
adhesion G protein-coupled receptor E1

UniProt Comments for F4/80

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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