PML Antibody – Mouse Monoclonal

PML: a zinc-finger protein that can regulate transcription and can localize to nuclear bodies. Cytoplasmic forms regulate glycolysis by inhibiting the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Sumoylated forms interact with SATB1 and localize to the PML nuclear bodies. Sumoylation on three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The B1 box and the RING finger are also required for localization to nuclear bodies. May play an important role in recruitment of ELF4 into PML nuclear bodies. A chromosomal aberration involving PML can cause acute promyelocytic leukemia (APL). Seven alternatively-spliced human isoforms have been reported.

Protein type: Nucleolus; Oncoprotein; Transcription factor; Tumor suppressor; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9 B|9 31.63 cM

Cellular Component: cytoplasm; cytosol; nuclear chromosome, telomeric region; nuclear matrix; nuclear membrane; nucleolus; nucleoplasm; nucleus; PML body

Molecular Function: protein binding; protein homodimerization activity; SMAD binding; SUMO binding; transcription coactivator activity; ubiquitin protein ligase binding

Biological Process: caspase activation; cell aging; cell cycle arrest; cell fate commitment; circadian regulation of gene expression; common-partner SMAD protein phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; endoplasmic reticulum calcium ion homeostasis; entrainment of circadian clock by photoperiod; induction of apoptosis by oxidative stress; innate immune response; maintenance of protein localization in nucleus; myeloid cell differentiation; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-1 secretion; negative regulation of transcription, DNA-dependent; PML body organization and biogenesis; positive regulation of fibroblast proliferation; positive regulation of MHC class I biosynthetic process; positive regulation of telomere maintenance; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; regulation of cell adhesion; regulation of circadian rhythm; regulation of MHC class I biosynthetic process; regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to gamma radiation; response to hypoxia; response to UV; retinoic acid receptor signaling pathway; SMAD protein nuclear translocation; transforming growth factor beta receptor signaling pathway