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CD27 Recombinant Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488454

Recombinant monoclonal antibody to CD27. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma LG.3A10. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD27, Recombinant Antibody
Full Product Name
Anti-CD27 [LG.3A10]
Product Gene Name
Product Synonym Names
TNFRS7; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7
Clonality
Monoclonal
Isotype
Hamster (Armenian) IgG kappa
Clone Number
LG.3A10
Species Reactivity
Mouse
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Protein A Affinity Purified
Concentration
1mg/ml (lot specific)
Specificity
This antibody is specific for mouse CD27, which, as in the human system, is largely restricted to cells of the T cell lineage, and is expressed on the great majority of both alphabeta and gammadelta T lymphocytes, and thymocytes.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
3D Structure
ModBase 3D Structure for P41272
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Antibody Species
Armenian Hamster
Original Species Name
Armenian Hamster
Immunogen
This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant.
Clone Note
This antibody is specific for murine CD27, and has been used to assess the distribution of CD27 expression using IF, FC and IP analysis (Gravstein, 1995). Upon cross-linking, this antibody has been shown to induce a 4-fold increase in T cell proliferation in response to suboptimal stimulation with concanavalin A (Gravestein, 1995), indicating that this antibody is able to mimic ligand binding.
Buffer Composition
PBS only with 0.02% Proclin 300.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for CD27 recombinant antibody
Activating, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Immunofluorescence (IF) of CD27 recombinant antibody

Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.
Anti-CD27 Monoclonal Recombinant Antibody – Cat# MBS488454

Testing Data of CD27 recombinant antibody

Binding curve of anti-CD27 antibody LG.3A10 to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.
Anti-CD27 Monoclonal Recombinant Antibody, Testing Data – Cat# MBS488454

Product References and Citations for CD27 recombinant antibody

Gravstein et al. Novel mAbs reveal potent co-stimulatory activity of murine CD27 International immunology, April 1995, Vol.7(4), pp.551-7 PMID:7547681
Original Published Journal: International immunology, April 1995, Vol.7(4), pp.551-7
Original Published Journal: International immunology, April 1995, Vol.7(4), pp.551-7
Publication Note: Describes the original use of this antibody to elucidate the distribution and co-stimulatory activity of CD27.

Other Notes

Small volumes of CD27 recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD27 purchase

MBS488454 is a recombinant antibody targeting CD27. Also known as Anti-CD27 [LG.3A10]. Reactive in Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 antigen
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
S152; Tp55; Tnfrsf7
UniProt Protein Name
CD27 antigen
UniProt Synonym Protein Names
CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27
Protein Family
UniProt Gene Name
Cd27
UniProt Synonym Gene Names
Tnfrsf7
UniProt Entry Name
CD27_MOUSE
NCBI Protein Information
CD27 antigen

UniProt Comments for CD27

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis

Cellular Component: cell surface; membrane; cell; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; caspase inhibitor activity

Biological Process: B cell proliferation; positive regulation of T cell differentiation; apoptosis; immunoglobulin secretion; positive regulation of JNK cascade; negative regulation of caspase activity; negative regulation of apoptosis; positive regulation of interferon-gamma biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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