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CD28 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800823

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells… More details

$265 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
Anti-Rt CD28 Purified
Product Gene Name
Product Synonym Names
CD28 molecule; Tp44
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
JJ319
Host
Mouse
Species Reactivity
Rat
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD28 Clone #JJ319 (MBS1800823)
FITC Conjugated Antibody: CD28 Clone #JJ319 (MBS1800824)
Matching Pairs
Unconjugated Antibody: CD28 Clone #JJ319 (MBS1800823)
PE Conjugated Antibody: CD28 Clone #JJ319 (MBS1800825)
3D Structure
ModBase 3D Structure for P31042
Immunogen
Mouse A20J B lymphoma cells transfected with rat CD28
Applications Tested/Suitable for anti-CD28 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes for anti-CD28 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD28 antibody

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 ug/ml) GAM APC.
Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS1800823

Flow Cytometry (FC/FACS) of anti-CD28 antibody

Flow cytometry surface staining pattern of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 ug/ml) GAM APC.
Mouse Anti-CD28 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800823

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD28 purchase

MBS1800823 is an antibody targeting CD28. Also known as Anti-Rt CD28 Purified. Reactive in Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28
NCBI Official Synonym Full Names
Cd28 molecule
NCBI Official Symbol
Cd28
NCBI Official Synonym Symbols
CD28RNA
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
CD_antigen: CD28
UniProt Gene Name
Cd28
UniProt Entry Name
CD28_RAT
NCBI Protein Information
T-cell-specific surface glycoprotein CD28

NCBI Summary for CD28

costimulatory molecule involved in induction of T cell proliferation and initiation of the Th2 response; may be important in maintanence of basal IgE production; binds to B7-1 or B7-2 on antigen presenting cells (APCs) [RGD, Feb 2006]

UniProt Comments for CD28

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CD28 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >262 publications with CD28 and Heart
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