FGF-2 (Basic) (FGF-2) Mouse Active Protein

FGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells (5). The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic (6,7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

1. Coulier F et al, J Mol Evol 44:43, 1997 2. Fernig D et al, Prog Growth Factor Res 5:353, 1994 3. Presta M et al, Cytokine Growth Factor Rev 16:159, 2005 4. Claus P et al, J Biol Chem 278:479, 2003 5. Quarto N et al, Gene 356:49, 2005 6. Tsuneto M et al, Biochem Biophys Res Comm 335:1239, 2005 7. Hebert JM et al, Dev Biol 138:454, 1990

Small volumes of FGF-2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS691614 is an active protein targeting FGF-2. Also known as Mouse FGF-2 (basic). Reactive in Mouse. Host: E Coli.